{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T02:00:29Z","timestamp":1789610429306,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,3,23]],"date-time":"2006-03-23T00:00:00Z","timestamp":1143072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:title>Resume<\/jats:title><jats:p><jats:bold>Objectifs\u2002<\/jats:bold> Evaluer la validit\u00e9, le co\u00fbt et la faisabilit\u00e9 de deux tests parasitologiques pour la confirmation de la maladie du sommeil; la mini Anion Exchange Centrifugation Technique (mAECT) et la Capillary Tube Centrifugation (CTC).<\/jats:p><jats:p><jats:bold>M\u00e9thodes<\/jats:bold> Au cours d'une campagne de d\u00e9pistage de la maladie du sommeil en 2004 nous avons examin\u00e9 6502 personnes \u00e0 Kwamouth en R\u00e9publique D\u00e9mocratique du Congo (RDC). Les personnes test\u00e9es positives au Card Agglutination Test for Trypanosomiasis sur sang total (CATT) ont re\u00e7u une ponction ganglionnaire, un examen de sang frais, une goutte \u00e9paisse color\u00e9e, un examen de mAECT, un examen de CTC et une titration de CATT. La sensibilit\u00e9 et sp\u00e9cificit\u00e9 des tests de confirmation ont \u00e9t\u00e9 calcul\u00e9es en utilisant la combinaison de tous les tests parasitologiques comme r\u00e9f\u00e9rence standard. Le co\u00fbt de chaque m\u00e9thode a \u00e9t\u00e9 calcul\u00e9 et leur faisabilit\u00e9 a \u00e9t\u00e9\u00e9valu\u00e9e par des interviews structur\u00e9es avec les techniciens.<\/jats:p><jats:p><jats:bold>R\u00e9sultats\u2002<\/jats:bold> La sensibilit\u00e9 des m\u00e9thodes parasitologiques classiques \u00e9tait de 44,8% (IC 95%: 36,8\u201353,0), pour la CTC 56,5% (IC 95%: 48,3\u201364,5) pour la mAECT 75,3 % (IC 95%: 67,7\u201381,9). Le co\u00fbt par test \u00e9tait de 2,82\u20ac pour la mAECT et 0,76\u20ac pour la CTC. Le temps pour l'obtention du r\u00e9sultat \u00e9tait de 29,78\u2003min pour la mAECT et 18,25\u2003min pour la CTC. Ces deux tests ont \u00e9t\u00e9 jug\u00e9s faisables sous les conditions de terrain.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> L'utilisation individuelle ou en combinaison de la CTC et de la mAECT comme tests de confirmation sur les personnes au CATT sur sang total apporterait une am\u00e9lioration consid\u00e9rable de la d\u00e9tection des cas de trypanosomiase humaine africaine. Les deux tests se sont av\u00e9r\u00e9s faisables dans des conditions op\u00e9rationnelles lorsque la disponibilit\u00e9 d'un courant de 220\u2003V pouvait \u00eatre garantie. Vue que le test mAECT est plus sensible mais consid\u00e9rablement plus co\u00fbteux, les analyses de co\u00fbt\u2010efficacit\u00e9 de la faisabilit\u00e9 devraient guider au choix du meilleur algorithme.<\/jats:p>","DOI":"10.1111\/j.1365-3156.2006.01591.x","type":"journal-article","created":{"date-parts":[[2006,3,23]],"date-time":"2006-03-23T11:01:03Z","timestamp":1143111663000},"page":"470-478","source":"Crossref","is-referenced-by-count":21,"title":["Validit\u00e9, co\u00fbt et faisabilit\u00e9 de la mAECT et CTC comme tests de confirmation dans la d\u00e9tection de la Trypanosomiase Humaine Africaine"],"prefix":"10.1111","volume":"11","author":[{"given":"P.","family":"Lutumba","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Robays","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Miaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Kande","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Mumba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"B\u00fcscher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Dujardin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Boelaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,3,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0258(19991130)18:22<2987::AID-SIM205>3.0.CO;2-B"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1177\/004947559402400204","article-title":"The quantitative buffy coat for the diagnosis of trypanosomes","volume":"24","author":"Bailey JM","year":"1994","journal-title":"Tropical Doctor"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.2001.00710.x"},{"key":"e_1_2_6_5_1","volume-title":"Guide technique du Programme national de lutte contre la THA en RD CONGO","author":"Bureau central de la trypanosomiase","year":"1995"},{"key":"e_1_2_6_6_1","volume-title":"D\u00e9claration de la Politique nationale de lutte contre la trypanosomiase humaine africaine en R\u00e9publique d\u00e9mocratique du Congo","author":"Bureau central de la trypanosomiase","year":"2002"},{"key":"e_1_2_6_7_1","first-page":"83","article-title":"Human African Trypanosomiasis: use of double centrifugation of cerebrospinal fluid to detect trypanosomes","volume":"66","author":"Cattand P","year":"1988","journal-title":"Bulletin of the World Health Organization"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"313","DOI":"10.4269\/ajtmh.2004.71.313","article-title":"Card agglutination test for trypanosomiasis (CATT) end\u2010dilution titer and cerebrospinal fluid cell count as predictors of human African Trypanosomiasis (Trypanosoma brucei gambiense) among serologically suspected individuals in southern Sudan","volume":"71","author":"Chappuis F","year":"2004","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.2000.00623.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/biomet\/61.2.215"},{"key":"e_1_2_6_11_1","first-page":"79","article-title":"Evaluation du diagnostic sur le terrain de la trypanosomiase \u00e0Trypanosoma brucei gambiense","volume":"61","author":"Henry MC","year":"1981","journal-title":"Annales de la Soci\u00e9t\u00e9 belge de M\u00e9decine Tropicale"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(79)90092-0"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2005.01391.x"},{"key":"e_1_2_6_14_1","first-page":"169","article-title":"A card\u2010agglutination test with stained trypanosomes (C. 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