{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T16:08:12Z","timestamp":1770480492488,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,8,8]],"date-time":"2006-08-08T00:00:00Z","timestamp":1154995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Objective\u2002<\/jats:bold> Differential diagnosis of infections that cause similar diseases and may be active simultaneously in the same geographical areas is greatly needed. Dengue and yellow fever viruses (DENV and YFV) are transmitted by the same species of mosquito and both can cause haemorrhagic fever symptoms. These viruses are active mainly in regions where expensive and sophisticated technologies are not available. Our objective was to develop a simple, reliable and easy\u2010to\u2010perform method to detect and identify these viruses.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> We slightly modified a generic RT\u2010PCR able to detect the mentioned viruses and other members of this genus: specific primers for each one of these viruses were designed and included in the nested reaction instead of one of the generic ones. The reaction was optimized and viruses are amplified giving rise to bands of different sizes distinguishable in agarose gels.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> This test is able to detect and identify the four DENVs and YFV to a high level of sensitivity and specificity and can be used with clinical samples. This simple, reliable and easy\u2010to\u2010perform method able to detect and identify dengue 1\u20134 and YFV can be used in poor endemic countries.<\/jats:p>","DOI":"10.1111\/j.1365-3156.2006.01696.x","type":"journal-article","created":{"date-parts":[[2006,8,8]],"date-time":"2006-08-08T08:01:19Z","timestamp":1155024079000},"page":"1432-1441","source":"Crossref","is-referenced-by-count":16,"title":["Detection and subtyping of dengue 1\u20134 and yellow fever viruses by means of a multiplex RT\u2010nested\u2010PCR using degenerated primers"],"prefix":"10.1111","volume":"11","author":[{"given":"M. P.","family":"S\u00e1nchez\u2010Seco","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Rosario","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Domingo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Vald\u00e9s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. G.","family":"Guzm\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Tenorio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,8,8]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"Manual de procedimientos de t\u00e9cnicas para el diagn\u00f3stico del dengue. Organizaci\u00f3n Panamericana de la Salud","author":"Balmaseda A","year":"2002"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2005.05.006"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2004.08.008"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2006.02.005"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.7.2323-2330.2002"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-3542(02)00201-2"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.324.7353.1563"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(01)00171-2"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(03)00010-6"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2003.03.003"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","first-page":"2634","DOI":"10.1128\/JCM.36.9.2634-2639.1998","article-title":"Typing of dengue viruses in clinical specimens and mosquitoes by single\u2010tube multiplex reverse transcriptase PCR","volume":"36","author":"Harris E","year":"1998","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.3.4.376"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"418","DOI":"10.4269\/ajtmh.1991.45.418","article-title":"Sensitivity and specificity of a universal primer set for the rapid diagnosis of Dengue virus infections by polymerase chain reaction and nucleic acid hybridisation","volume":"45","author":"Henchal EA","year":"1991","journal-title":"The American Journal of Tropical Medicine and Hygiene"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(01)00280-4"},{"key":"e_1_2_6_16_1","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1128\/jcm.30.3.545-551.1992","article-title":"Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase\u2010polymerase chain reaction","volume":"30","author":"Lanciotti RS","year":"1992","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0904.020267"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1997.tb01192.x"},{"key":"e_1_2_6_19_1","first-page":"109","article-title":"The role of Aedes albopictus as an arbovirus vector","volume":"37","author":"Mitchell CJ","year":"1995","journal-title":"Parassitologia"},{"key":"e_1_2_6_20_1","first-page":"143","volume-title":"Factor in the Emergence of Arbovirus Diseases","author":"Monath TP","year":"1997"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(01)00016-0"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","first-page":"2107","DOI":"10.1128\/jcm.29.10.2107-2110.1991","article-title":"Rapid identification of dengue virus serotypes by using polymerase chain reaction","volume":"29","author":"Morita K","year":"1991","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2895-2903.2005"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(02)00104-0"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10465"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2005.01.025"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340090304"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(94)00104-O"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.11.4.642-650.2004"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-002-3052-7"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(93)90020-R"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.461"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.2310\/7060.2004.18503"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(01)00250-5"}],"container-title":["Tropical Medicine &amp; International Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-3156.2006.01696.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-3156.2006.01696.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T05:06:29Z","timestamp":1696914389000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-3156.2006.01696.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,8,8]]},"references-count":33,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2006,9]]}},"alternative-id":["10.1111\/j.1365-3156.2006.01696.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-3156.2006.01696.x","archive":["Portico"],"relation":{},"ISSN":["1360-2276","1365-3156"],"issn-type":[{"value":"1360-2276","type":"print"},{"value":"1365-3156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,8,8]]}}}