{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:09:31Z","timestamp":1791202171285,"version":"4.2.0"},"reference-count":12,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,3,3]],"date-time":"2011-03-03T00:00:00Z","timestamp":1299110400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tropical Med Int Health"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Objective\u2002<\/jats:bold> To identify the aetiological agent\/s of an outbreak of chikungunya\u2010like illness with high morbidity and several fatalities in Tamil Nadu, India, 2009\u20132010.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Two hundred and seventeen serum samples were collected from the affected areas and screened for chikungunya virus (CHIKV), dengue virus (DENV) and Japanese encephalitis virus (JEV) IgM antibodies using MAC\u2010ELISA kits. A few selected samples were also tested for Ross River, Sindbis, and Murrey Valley viruses by RT\u2010PCR and Hantan virus by serology. Twelve acute serum and mosquito samples were processed for virus isolation in C6\/36 cells. CHIKV isolate was characterised by RT\u2010PCR and sequencing.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Diagnostic levels of IgM antibodies were detected in 107 (49.3%) CHIKV samples and 22 (10.1%) DENV samples. IgM antibodies against JEV were not detected (<jats:italic>n<\/jats:italic>\u2003=\u200346). Characterisation of the CHIKV isolate at genetic level demonstrated it as ECSA (E1: 226A). Thirty\u2010six selected samples were also negative for Ross River, Sindbis, Murrey Valley and Hantan viruses.<\/jats:p><jats:p><jats:bold>Conclusion\u2002<\/jats:bold> High prevalence of CHIKV IgM antibody positivity, clinical symptoms, virus isolation and the presence of vector mosquitoes clearly suggest CHIKV as the aetiological agent responsible for the outbreak.<\/jats:p>","DOI":"10.1111\/j.1365-3156.2011.02743.x","type":"journal-article","created":{"date-parts":[[2011,3,3]],"date-time":"2011-03-03T21:37:28Z","timestamp":1299188248000},"page":"585-588","source":"Crossref","is-referenced-by-count":6,"title":["Investigation of a Chikungunya\u2010like illness in Tirunelveli district, Tamil Nadu, India 2009\u20132010"],"prefix":"10.1111","volume":"16","author":[{"given":"A. B.","family":"Sudeep","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. L.","family":"Hundekar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. G.","family":"Jacob","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Balasubramanian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. A.","family":"Arankalle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. C.","family":"Mishra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,3,3]]},"reference":[{"key":"e_1_2_3_2_1","first-page":"1","article-title":"Fauna of British India including Ceylon and Burma","volume":"5","author":"Barraud PJ","year":"1934","journal-title":"Diptera"},{"key":"e_1_2_3_3_1","first-page":"764","article-title":"Chikungunya disease in infants and children in Vellore: a report of clinical and hematological features of virologically proved cases","volume":"53","author":"Jadhav M","year":"1965","journal-title":"Indian Journal of Medical Research"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02724120"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1410.070569"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.83628-0"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2010.01.011"},{"key":"e_1_2_3_8_1","first-page":"101","article-title":"West Nile virus: the Indian scenario","volume":"118","author":"Paramasivan R","year":"2003","journal-title":"Indian Journal of Medical Research"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-6-172"},{"key":"e_1_2_3_10_1","first-page":"684","article-title":"Virological and serological studies of cases of hemorrhagic fever in Calcutta. Material collected by the Calcutta school of tropical medicine","volume":"52","author":"Sarkar JK","year":"1964","journal-title":"Indian Journal of Medical Research"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030201"},{"key":"e_1_2_3_12_1","first-page":"112","article-title":"Laboratory based dengue fever surveillance in Tamil Nadu, India","volume":"126","author":"Victor TJ","year":"2007","journal-title":"Indian Journal of Medical Research"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1210.060529"}],"container-title":["Tropical Medicine &amp; International Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-3156.2011.02743.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-3156.2011.02743.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T18:11:21Z","timestamp":1698775881000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-3156.2011.02743.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3,3]]},"references-count":12,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2011,5]]}},"alternative-id":["10.1111\/j.1365-3156.2011.02743.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-3156.2011.02743.x","archive":["Portico"],"relation":{},"ISSN":["1360-2276","1365-3156"],"issn-type":[{"value":"1360-2276","type":"print"},{"value":"1365-3156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,3,3]]}}}