{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:44:58Z","timestamp":1791287098174,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,11,6]],"date-time":"2006-11-06T00:00:00Z","timestamp":1162771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sarcoptic mange occurs in many parts of the world and is common in populations of domestic and wild canids, including red foxes (<jats:italic>Vulpes vulpes<\/jats:italic>). In recent years, an indirect antibody enzyme\u2010linked immunosorbent assay (ELISA), with higher sensitivity and specificity than traditional diagnostic methods, has been successfully applied in the diagnosis of sarcoptic mange in dogs. The same ELISA has also demonstrated specific antibodies to<jats:italic>Sarcoptes scabiei<\/jats:italic>in experimentally infected red foxes. The aim of this study was to evaluate the indirect ELISA when used to detect antibodies to<jats:italic>S. scabiei<\/jats:italic>in field sera from Swedish red foxes. One cohort of both infected and non\u2010infected red foxes (cohort 1;<jats:italic>n<\/jats:italic>\u00a0=\u00a088), and one cohort of apparently non\u2010infected foxes (cohort 2;<jats:italic>n<\/jats:italic>\u00a0=\u00a067) were examined for skin lesions and presence of<jats:italic>S. scabiei<\/jats:italic>by thorough visual examination at autopsy and skin scrapings. Samples of blood\u2010tinted body liquid from the abdomen or thorax cavity were collected and analysed by the indirect ELISA. The relative sensitivity and specificity of the ELISA at different cut\u2010offs (OD values) were estimated by comparing the test results to the infection status as determined by examination and skin scrapings. The highest combination of relative sensitivity and specificity, calculated based on cohort 1, was 95.4 and 100.0%, respectively. These estimates were constant for cut\u2010offs 0.150\u20130.225, which included the cut\u2010off based on the mean plus three standard deviations of test results from cohort 2 (0.165). It is concluded that this test can be useful in diagnosis and epidemiological studies of<jats:italic>S. scabiei<\/jats:italic>infection in red foxes.<\/jats:p>","DOI":"10.1111\/j.1365-3164.2006.00548.x","type":"journal-article","created":{"date-parts":[[2006,11,6]],"date-time":"2006-11-06T09:36:30Z","timestamp":1162805790000},"page":"411-416","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Evaluation of a serological test (indirect ELISA) for the diagnosis of sarcoptic mange in red foxes (<i>Vulpes vulpes<\/i>)"],"prefix":"10.1111","volume":"17","author":[{"given":"Set","family":"Bornstein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Fr\u00f6ssling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarina","family":"N\u00e4slund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00f6ran","family":"Zakrisson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten","family":"M\u00f6rner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,11,6]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780470377000.ch5"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(99)00034-X"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-002-0655-y"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.20506\/rst.21.2.1335"},{"key":"e_1_2_7_6_2","first-page":"12","article-title":"Sarcoptic mange in red fox in Denmark. A short report","volume":"76","author":"Henriksen P","year":"1993","journal-title":"Dansk Veterin\u00e4rtidskrift"},{"key":"e_1_2_7_7_2","first-page":"233","article-title":"The epizootiology of sarcoptic mange in chamois, Rupricapra rupricapra, from the Italian Eastern Alps","volume":"37","author":"Rossi L","year":"1995","journal-title":"Parassitologica (Roma)"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(97)00061-7"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-0813.1998.tb12391.x"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.150.1.12"},{"key":"e_1_2_7_11_2","unstructured":"M\u00f6rnerT UhlhornH Br\u00f6jerCet al.The introduction of sarcoptic mange in the red fox (Vulpes vulpes) population in Sweden and its ecological consequences \u2013 30 years experience 2005.International Conference of the International Wildlife DiseaseJune 26July 1st 2005 Cairns Australia. Proceedings 54."},{"key":"e_1_2_7_12_2","unstructured":"BornsteinS.Sarcoptes scabieiinfections of the domestic dog red fox and pig: clinical and serodiagnostic studies.Doctoral Thesis.Uppsala Sweden: Swedish University of Agricultural Sciences and the National Veterinary Institute 1995."},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/BF03547665"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.1996.tb00222.x"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.141.1.8"},{"key":"e_1_2_7_16_2","first-page":"183","article-title":"Demonstration of serum antibody to Sarcoptes scabiei in naturally infected camels. 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