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Twenty\u2010one atopic dogs without clinical and\/or cytopathological signs of bacterial blepharoconjunctivitis and 21 breed\u2010matched healthy dogs were enrolled. Under topical anaesthesia, the inferior conjunctival sac of one eye was scraped twice. Material was collected with a Kimura spatula, spread over a slide and stained with a Diff Quick<jats:sup>\u00ae<\/jats:sup>\u2010type stain (Medion Diagnostics GmbH, D\u00fcdingen, Switzerland) for cytological examination. An area of 0.5\u2003cm<jats:sup>2<\/jats:sup>was examined at \u00d71000 magnification, and the types and numbers of cells and bacteria were recorded. A bacterial swab was collected and inoculated into culture media for the growth of aerobic bacteria. Before sampling, each atopic dog was evaluated for severity of cutaneous lesions, pruritus and conjunctival inflammation. Significant differences were observed between atopic and healthy dogs for the presence of bacteria on cytology (<jats:italic>P<\/jats:italic>\u2003=\u20030.015), keratinized (<jats:italic>P\u2003<\/jats:italic>= 0.001) and nonkeratinized epithelial cells (<jats:italic>P\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.013), eosinophils (<jats:italic>P\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.019) and lymphocytes (<jats:italic>P\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.008). Bacteria were recovered from 12 atopic dogs and three healthy dogs (<jats:italic>P\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.004).<jats:italic>Staphylococcus pseudintermedius<\/jats:italic>was the most commonly isolated species in atopic dogs (seven of 12). In atopic dogs, no significant relation was found between conjunctival bacterial colonization (on cytology and culture) and the severity of any of the clinical parameters. This study suggests differences in conjunctival bacterial colonization and cytological features between atopic and healthy dogs.<\/jats:p>","DOI":"10.1111\/j.1365-3164.2011.00979.x","type":"journal-article","created":{"date-parts":[[2011,5,2]],"date-time":"2011-05-02T08:56:30Z","timestamp":1304326590000},"page":"490-496","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Evaluation of the bacterial microflora of the conjunctival sac of healthy dogs and dogs with atopic dermatitis"],"prefix":"10.1111","volume":"22","author":[{"given":"Nicla","family":"Furiani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabia","family":"Scarampella","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piera","family":"Anna 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