{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T10:28:46Z","timestamp":1761128926308},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Chinese shar\u2010pei dog is known for its distinctive feature of wrinkled and thickened skin, defined as primary or hereditary cutaneous mucinosis. In a recent report, we identified the mucinous material deposited in the shar\u2010pei skin as the polysaccharide hyaluronan (HA). In the present work, the molecular and cellular mechanisms underlying this phenotype have been identified in dermal fibroblasts isolated from shar\u2010pei dogs. The production of HA, which appeared to be mainly associated with cell membrane protrusions and also intracellular, was higher in shar\u2010pei fibroblasts than in control cells. The HA accumulation is related to a higher mRNA expression of the isoform <jats:italic>HAS2<\/jats:italic> of the HA\u2010synthesizing enzyme family, hyaluronan synthases (HAS). The higher expression of <jats:italic>HAS2<\/jats:italic> in shar\u2010pei fibroblasts was confirmed at the protein level. The other HAS isoenzymes, <jats:italic>HAS1<\/jats:italic> and <jats:italic>HAS3<\/jats:italic>, and the HA\u2010degrading enzymes, <jats:italic>Hyal1<\/jats:italic> and <jats:italic>Hyal2<\/jats:italic>, were not differentially expressed in shar\u2010pei fibroblasts compared with cells from control dogs. Fibroblasts from shar\u2010pei dogs and from control dogs are morphologically different as observed by transmission electron microscopy. Scanning electron microscopy revealed a large number of cellular protrusions with associated globular deposits. Electron microscopy after labelling with biotinylated HA\u2010binding protein confirmed an increased HA content in shar\u2010pei fibroblasts, which could be localized in several subcellular structures. The authors propose the name hereditary cutaneous hyaluronosis (HCH) for affected dogs, because it better defines the cutaneous mucinosis of shar\u2010pei dogs.<\/jats:p>","DOI":"10.1111\/j.1365-3164.2011.00986.x","type":"journal-article","created":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T09:41:05Z","timestamp":1309513265000},"page":"535-545","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Increased HAS2\u2010driven hyaluronic acid synthesis in shar\u2010pei dogs with hereditary cutaneous hyaluronosis (mucinosis)"],"prefix":"10.1111","volume":"22","author":[{"given":"Mar\u00eda Jos\u00e9","family":"Docampo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giordana","family":"Zanna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dolors","family":"Fondevila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Cabrera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"L\u00f3pez\u2010Iglesias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santiago","family":"Cerrato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Llu\u00eds","family":"Ferrer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Bassols","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,7]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"The Chinese Shar Pei","author":"Strang PC","year":"1980"},{"key":"e_1_2_6_3_2","first-page":"189","article-title":"Idiopathic mucinosis cutis in Chinese shar\u2010pei dogs: epidemiology, clinical features, histopathologic findings and treatment","volume":"26","author":"von Bomhard D","year":"1998","journal-title":"Tier\u00e4rztliche Praxis Ausgabe K, Kleintiere\/Heimtiere"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2008.00703.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1391"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2007.08.008"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2007.09.013"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2009.00799.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909918107"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2006.00525.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.68.12.1376"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20061757"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2008.951665"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2009.953802"},{"key":"e_1_2_6_16_2","first-page":"3261","article-title":"Expression regulation of hyaluronan synthase in corneal endothelial cells","volume":"41","author":"Usui T","year":"2000","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M504736200"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R700036200"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.012492"},{"key":"e_1_2_6_20_2","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1042\/bj3480029","article-title":"Expression of human hyaluronan synthases in response to external stimuli","volume":"348","author":"Jacobson A","year":"2000","journal-title":"Biochemical Journal"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M512840200"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.19.4.1004"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1177\/002215549904701013"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2004.02.013"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.4A6356.2004"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M011004200"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.6.7.1563592"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003030200"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(92)90057-U"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jhered.a111369"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.080831"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.081138"},{"key":"e_1_2_6_33_2","first-page":"996","volume-title":"Muller and Kirk\u2019s Small Animal Dermatology","author":"Scott DW","year":"2001"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keh014"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2009.210"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(00)90051-9"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-3164.2011.00986.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-3164.2011.00986.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T01:18:41Z","timestamp":1697246321000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-3164.2011.00986.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7]]},"references-count":35,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["10.1111\/j.1365-3164.2011.00986.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-3164.2011.00986.x","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,7]]},"assertion":[{"value":"2011-07-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}