{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T05:30:36Z","timestamp":1789882236463,"version":"4.0.1"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,11,23]],"date-time":"2011-11-23T00:00:00Z","timestamp":1322006400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Gram\u2010positive bacterium <jats:italic>Staphylococcus pseudintermedius<\/jats:italic> is regarded as the major cause of canine bacterial pyoderma. Despite its clinical importance, there is only very limited knowledge about the pathogenesis of <jats:italic>S.\u00a0pseudintermedius<\/jats:italic> infection and the specific bacterial virulence factors involved in causing disease. Using a whole\u2010genome approach, we have previously identified 18 predicted cell\u2010wall\u2010anchored surface proteins representing possible virulence factors in a clinical isolate of <jats:italic>S.\u00a0pseudintermedius<\/jats:italic> (strain ED99). They were designated <jats:italic>S.\u00a0pseudintermedius<\/jats:italic> surface proteins A\u2013R (SpsA\u2013SpsR). The present study tested three of the putative Sps proteins (SpsD, SpsL and SpsO) for their ability to mediate adherence of bacteria to canine corneocytes. The three proteins were expressed on the surface of the nonpathogenic surrogate host <jats:italic>Lactococcus lactis<\/jats:italic>, a Gram\u2010positive bacterium that does not adhere to canine corneocytes. Adherence assays were performed using corneocytes from different healthy canine donors (<jats:italic>n\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>5), and bacterial cells were quantified using computerized image analysis. Two of the proteins, SpsD and SpsO, mediated adherence of <jats:italic>L.\u00a0lactis<\/jats:italic> to canine corneocytes, suggesting that they contribute to <jats:italic>S.\u00a0pseudintermedius<\/jats:italic> pathogenesis and may represent novel therapeutic targets to combat infection.<\/jats:p>","DOI":"10.1111\/j.1365-3164.2011.01021.x","type":"journal-article","created":{"date-parts":[[2011,11,23]],"date-time":"2011-11-23T15:16:47Z","timestamp":1322061407000},"page":"119","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["<i>Staphylococccus pseudintermedius<\/i> surface proteins SpsD and SpsO mediate adherence to <i>ex vivo<\/i> canine corneocytes"],"prefix":"10.1111","volume":"23","author":[{"given":"Jeanette","family":"Bannoehr","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy K.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darren J.","family":"Shaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ross J.","family":"Fitzgerald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adri H. M.","family":"van den Broek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keith L.","family":"Thoday","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,11,23]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"Small Animal Dermatology","author":"Patel A","year":"2008"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.06.002"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/519254"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1053\/rvsc.2000.0378"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0959-4493.2001.00269.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3164.2002.00294.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2004.09.002"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2005.00452.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2005.00484.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2006.00503.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2007.00567.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2007.00649.x"},{"key":"e_1_2_6_14_2","first-page":"270","volume-title":"Advances in Veterinary Dermatology","author":"Simou C","year":"2005"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(98)01400-0"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.1995.tb00418.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.03.030"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00137-11"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00137-11"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01150-07"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3516-3522.2000"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2002.00231.x"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M408713200"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0050017"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-22"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3164.1997.d01-5.x"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01304-08"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M610940200"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2007.11.006"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.23587.x"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2008.00707.x"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-3164.2011.01021.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-3164.2011.01021.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T12:03:44Z","timestamp":1697285024000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-3164.2011.01021.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11,23]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1111\/j.1365-3164.2011.01021.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-3164.2011.01021.x","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11,23]]},"assertion":[{"value":"2011-11-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}