{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T02:12:28Z","timestamp":1777428748901,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,3,12]],"date-time":"2012-03-12T00:00:00Z","timestamp":1331510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p><jats:bold>Background\u2002\u2013\u2002<\/jats:bold>Meticillin\u2010resistant<jats:italic>Staphylococcus aureus<\/jats:italic>(MRSA) and meticillin\u2010resistant<jats:italic>Staphylococcus pseudintermedius<\/jats:italic>(MRSP) infections are increasingly reported in dogs, and these bacteria may be isolated from ear infections.<\/jats:p><jats:p><jats:bold>Hypothesis\/Objectives\u2002\u2013\u2002<\/jats:bold>The main aim of the present study was to investigate the<jats:italic>in vitro<\/jats:italic>antimicrobial activity of miconazole, polymyxin\u2003B and a combination of both against 24 canine MRSA and 50 canine MRSP isolates. The minimal inhibitory concentration (MIC) values of 12 other antimicrobial agents were also determined.<\/jats:p><jats:p><jats:bold>Methods\u2002\u2013\u2002<\/jats:bold>All MIC values were determined according to a broth microdilution assay.<\/jats:p><jats:p><jats:bold>Results\u2002\u2013\u2002<\/jats:bold>Acquired resistance was found to all tested agents, except for linezolid, miconazole and polymyxin\u2003B. The MIC values for miconazole and polymyxin\u2003B against MRSA were in the range of 4\u20138 and 8\u201364\u2003\u03bcg\/mL, respectively, while the MIC values for miconazole and polymyxin\u2003B against MRSP were in the range of 1\u20132 and 0.25\u20134\u2003\u03bcg\/mL, respectively. Using a combination of miconazole and polymyxin\u2003B, there was no evidence for enhanced<jats:italic>in vitro<\/jats:italic>activity of the combination (i.e. synergy) of both products. Nevertheless, MIC<jats:sub>90<\/jats:sub>values of the combination of these antimicrobial agents and of a commercial product containing both agents were at least 1000 times lower than the concentration present in the commercial product.<\/jats:p><jats:p><jats:bold>Conclusions and clinical importance\u2002\u2013\u2002<\/jats:bold>These results indicate that the topical use of a combination of miconazole and polymyxin\u2003B in a 43.5:1 ratio may have potential for the treatment of MRSA\u2010mediated and MRSP\u2010associated otitis externa in dogs.<\/jats:p>","DOI":"10.1111\/j.1365-3164.2012.01040.x","type":"journal-article","created":{"date-parts":[[2012,3,12]],"date-time":"2012-03-12T19:37:31Z","timestamp":1331581051000},"page":"381","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["<i>In vitro<\/i>antimicrobial activity of miconazole and polymyxin B against canine meticillin\u2010resistant<i>Staphylococcus aureus<\/i>and meticillin\u2010resistant<i>Staphylococcus pseudintermedius<\/i>isolates"],"prefix":"10.1111","volume":"23","author":[{"given":"Filip","family":"Boyen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koen M. 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