{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T19:40:08Z","timestamp":1766086808255},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,1,19]],"date-time":"2013-01-19T00:00:00Z","timestamp":1358553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:p><jats:bold>Background\u00a0\u2013\u00a0<\/jats:bold> Cefovecin has been widely used to treat skin infections in dogs. The relationship of the cefovecin disk\u2010diffusion test results to the presence of the <jats:italic>mecA<\/jats:italic> gene and the clinical efficacy of cefovecin have not been fully evaluated.<\/jats:p><jats:p><jats:bold>Hypothesis\/Objectives\u00a0\u2013\u00a0<\/jats:bold> To determine the usefulness of an <jats:italic>in vitro<\/jats:italic> cefovecin disk\u2010diffusion test in predicting the presence of the <jats:italic>mecA<\/jats:italic> gene in <jats:italic>Staphylococcus pseudintermedius<\/jats:italic>, as well as the <jats:italic>in vivo<\/jats:italic> efficacy of cefovecin therapy in dogs with superficial pyoderma.<\/jats:p><jats:p><jats:bold>Methods\u00a0\u2013\u00a0<\/jats:bold> Twenty\u2010six <jats:italic>S.\u00a0pseudintermedius<\/jats:italic> strains isolated from 22 dogs with pyoderma were used. <jats:italic>In vitro<\/jats:italic> disk\u2010diffusion test results of cefovecin were compared with agar\u2010dilution test results, the presence of the <jats:italic>mecA<\/jats:italic> gene, and the improvement in clinical scores of dogs with superficial pyoderma at 14\u2003days post treatment.<\/jats:p><jats:p><jats:bold>Results\u00a0\u2013\u00a0<\/jats:bold> There was a significant linear correlation (<jats:italic>r\u2003<\/jats:italic>=\u2003\u22120.83) between the diameter of the obvious zone of inhibition by disk diffusion and the minimal inhibitory concentration for cefovecin (<jats:italic>P\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0.0001). Receiver operating characteristic analysis revealed that zone diameters between 25 and 27\u2003mm exhibited better sensitivity (92.9%) and specificity (100.0%) for detection of strains carrying the <jats:italic>mecA<\/jats:italic> gene. The mean improvement in clinical scores in dogs carrying cefovecin\u2010resistant strains was significantly lower than in dogs carrying cefovecin\u2010susceptible strains (<jats:italic>P\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0.01).<\/jats:p><jats:p><jats:bold>Conclusions and clinical importance\u00a0\u2013\u00a0<\/jats:bold> The cefovecin disk\u2010diffusion test with a cut\u2010off value estimated in this study was valuable for predicting <jats:italic>mecA<\/jats:italic> gene carriage in <jats:italic>S.\u00a0pseudintermedius<\/jats:italic>, as well as the <jats:italic>in vivo<\/jats:italic> efficacy of cefovecin therapy in dogs with superficial pyoderma caused by <jats:italic>S.\u00a0pseudintermedius<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1365-3164.2012.01100.x","type":"journal-article","created":{"date-parts":[[2013,1,19]],"date-time":"2013-01-19T14:29:52Z","timestamp":1358605792000},"page":"162","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Usefulness of cefovecin disk\u2010diffusion test for predicting <i>mecA<\/i> gene\u2010containing strains of <i>Staphylococcus pseudintermedius<\/i> and clinical efficacy of cefovecin in dogs with superficial pyoderma"],"prefix":"10.1111","volume":"24","author":[{"given":"Keita","family":"Iyori","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoichi","family":"Toyoda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaori","family":"Ide","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiroh","family":"Iwasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Nishifuji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,19]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/B0-72-162571-1\/50005-2"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01150-07"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63413-0"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02193-06"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2885.2006.00801.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.233.3.433"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.2007.00363.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2008.00663.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.01.039"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063870902100108"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-002-0436-0"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3164.2007.00635.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.4.781"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.suppl_1.65"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.2736\/jjvd.14.71"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01232-09"},{"key":"e_1_2_6_18_2","unstructured":"Clinical and Laboratory Standards Institute.Performance standards for antimicrobial susceptibility testing; 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