{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T02:23:22Z","timestamp":1783736602331,"version":"3.55.0"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,1,19]],"date-time":"2013-01-19T00:00:00Z","timestamp":1358553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Dermatology"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:p><jats:bold>Background\u00a0\u2013\u2002<\/jats:bold> Seborrhoea is a clinical condition resulting in excessive lipid and\/or scale on the skin and is a common and important skin disease of dogs. However, there is little information on the skin surface lipid composition of dogs with seborrhoea.<\/jats:p><jats:p><jats:bold>Hypothesis\/Objectives\u00a0\u2013\u2002<\/jats:bold> To compare skin surface lipid profiles in normal and seborrhoeic shih tzu dogs.<\/jats:p><jats:p><jats:bold>Methods\u00a0\u2013\u2002<\/jats:bold> Fourteen client\u2010owned dogs (seven seborrhoeic and seven normal) were investigated. Lipids in sebaceous glands (SGs) were extracted from homogenized tissues of SG hyperplasia. Surface lipid was collected by tape stripping [stratum corneum (SC)\u2010enriched fraction] and acetone\u2010wetted cotton swab (acetone\u2010extracted fraction). Lipids in SGs, SC\u2010enriched fractions and acetone\u2010extracted fractions were evaluated by high\u2010performance thin\u2010layer chromatography.<\/jats:p><jats:p><jats:bold>Results\u00a0\u2013\u2002<\/jats:bold> Lipids in SGs mainly consisted of cholesterol esters, wax esters and triglycerides, whereas lipids in the SC\u2010enriched fraction mainly consisted of ceramides. The acetone\u2010extracted fraction contained a mixture of lipid classes recognized in SG\u2010 and SC\u2010enriched fractions. In seborrhoeic dogs, concentrations of wax esters and\u2003triglycerides in the acetone\u2010extracted fraction were significantly higher than in control dogs (<jats:italic>P\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.0285). Amounts of total ceramides (in micrograms) per milligram of SC were not significantly different between the two groups (<jats:italic>P\u2003<\/jats:italic>=<jats:italic>\u2003<\/jats:italic>0.5204). Interestingly, two unknown ceramide fractions, which accounted for 20% of the total ceramides, were recognized exclusively in seborrhoeic dogs.<\/jats:p><jats:p><jats:bold>Conclusions and clinical importance\u00a0\u2013\u2002<\/jats:bold> These results provide evidence that the skin surface lipid profiles are altered in shih tzu dogs with seborrhoea.<\/jats:p>","DOI":"10.1111\/j.1365-3164.2012.01102.x","type":"journal-article","created":{"date-parts":[[2013,1,19]],"date-time":"2013-01-19T14:29:52Z","timestamp":1358605792000},"page":"84","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Skin lipid profiling in normal and seborrhoeic shih tzu dogs"],"prefix":"10.1111","volume":"24","author":[{"given":"Ji\u2010Seon","family":"Yoon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Nishifuji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinpei","family":"Ishioroshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Ide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiroh","family":"Iwasaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,19]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.4161\/derm.1.2.7811"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.08.039"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2006.06.003"},{"key":"e_1_2_6_5_2","first-page":"1030","volume-title":"Muller and Kirk\u2019s Small Animal Dermatology","author":"Scott DW","year":"2000"},{"key":"e_1_2_6_6_2","first-page":"1918","article-title":"Cell proliferation kinetics of epidermis, hair follicles, and sebaceous glands of cocker spaniels with idiopathic seborrhea","volume":"50","author":"Kwochka KW","year":"1989","journal-title":"Am J Vet Res"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3164.1997.d01-15.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1139\/y59-099"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)32369-5"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2011.01306.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.70.841"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/321494"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700305"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12676800"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M800014-JLR200"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2009.12.032"},{"key":"e_1_2_6_17_2","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/S0022-2275(20)34214-0","article-title":"Role of ceramide\u20031 in the molecular organization of the stratum corneum lipids","volume":"39","author":"Bouwstra JA","year":"1998","journal-title":"J Lipid Res"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemphyslip.2009.10.007"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.3.120"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2003.12103.x"}],"container-title":["Veterinary Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-3164.2012.01102.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-3164.2012.01102.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T07:47:02Z","timestamp":1697269622000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-3164.2012.01102.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,19]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1111\/j.1365-3164.2012.01102.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-3164.2012.01102.x","archive":["Portico"],"relation":{},"ISSN":["0959-4493","1365-3164"],"issn-type":[{"value":"0959-4493","type":"print"},{"value":"1365-3164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,19]]},"assertion":[{"value":"2013-01-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}