{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T03:03:43Z","timestamp":1778555023340,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2004,11,2]],"date-time":"2004-11-02T00:00:00Z","timestamp":1099353600000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> To date the direct fresh examination has not been considered, except by a few authors, as a valid laboratory procedure for the diagnosis of cutaneous sporotrichosis.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Forty\u2010two patients with cutaneous sporotrichosis (36 lymphangitic and six fixed) were studied. Pus was obtained through digital compression of opened lesions followed by collection with a spatula and examination of the material between the slide and the coverslip. Cultures on Sabouraud's glucose agar slants with cloramphenicol and cicloheximide were made in all cases.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> Direct, fresh examination asteroid bodies of <jats:italic>Sporothrix schenckii<\/jats:italic> were found in 85.7% of the cases, which allowed the authors to immediately diagnose the disease. Cultures confirmed the diagnosis in 95.2% of the patients.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> Fastness, simplicity and low cost of the fresh examination lead us to recommend systematic use of the method for primary diagnosis of the disease, mainly at first level public health laboratories.<\/jats:p>","DOI":"10.1111\/j.1365-4632.2004.02271.x","type":"journal-article","created":{"date-parts":[[2004,11,2]],"date-time":"2004-11-02T14:24:10Z","timestamp":1099405450000},"page":"808-810","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Importance of the direct fresh examination in the diagnosis of cutaneous sporotrichosis"],"prefix":"10.1111","volume":"43","author":[{"given":"Eduardo S.","family":"Civila","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio","family":"Bonasse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ismael A.","family":"Conti\u2010D\u00edaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ra\u00fal A.","family":"Vignale","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,11,2]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"62","article-title":"Sobre a cultura de uma nova especie de cogumelo patog\u00eanica do homen","volume":"3","author":"Splendore A","year":"1908","journal-title":"Rev Soc Scien De Sao Paulo"},{"key":"e_1_2_6_3_2","first-page":"78","article-title":"Biolog\u00eda del Sporotrichum schenckii\u2010beurmanni","volume":"1","author":"Greco NV","year":"1908","journal-title":"Rev Derm Argent"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1051\/parasite\/1935136576","article-title":"Deux cas de Sporotrichose produits par le Sporotrichum asteroide de Splendore","volume":"13","author":"Talice RV","year":"1935","journal-title":"Anales Parasitologie Humaine Compar\u00e9e"},{"key":"e_1_2_6_5_2","unstructured":"TaliceRV MackinnonJE.The asteroid form of Splendore in spontaneous and experimental sporotrichosis.Proc Third Int Con Microb1940:510\u2013511."},{"key":"e_1_2_6_6_2","first-page":"51","article-title":"Esporotricosis. 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