{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T09:29:00Z","timestamp":1770888540054,"version":"3.50.1"},"reference-count":13,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,4,27]],"date-time":"2006-04-27T00:00:00Z","timestamp":1146096000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2006,5]]},"abstract":"Abstract<\/jats:title>Objective\u2002<\/jats:bold> To evaluate the role of the enzyme\u2010linked immunosorbent assay (ELISA) test for the detection of antibodies to desmoglein 1 (dsg1) and desmoglein 3 (dsg3) in the diagnosis of pemphigus vulgaris (PV), and its correlation with disease severity and clinical presentation (mucosal PV, cutaneous PV, mucocutaneous PV).<\/jats:p>Methods\u2002<\/jats:bold> Twenty\u2010seven active PV patients and 26 controls with other dermatologic disorders were included in the study. The severity of oral and cutaneous involvement was assessed and recorded. ELISA test for the measurement of anti\u2010dsg1 and anti\u2010dsg3 antibodies was performed (Medical and Biological Laboratories Co. Ltd., Nagoya, Japan). The cut\u2010off ELISA value for both anti\u2010dsg1 and anti\u2010dsg3 was taken as 20.<\/jats:p>Results\u2002<\/jats:bold> Of the 27 patients, 26 were ELISA positive for anti\u2010dsg1 antibodies and 23 for anti\u2010dsg3 antibodies. Of the controls, two were positive for anti\u2010dsg1 and none for anti\u2010dsg3 antibodies. The sensitivity and specificity of ELISA for anti\u2010dsg1 in the diagnosis of PV were 96.3% and 92.3%, respectively. For anti\u2010dsg3, they were 85.2% and 100%, respectively. The different morphologic types of PV could not be differentiated on the basis of antibody profile; however, a direct correlation between anti\u2010dsg3 titers and the severity of oral disease was noted, and also between anti\u2010dsg1 titers and the severity of cutaneous disease.<\/jats:p>Conclusions\u2002<\/jats:bold> ELISA (dsg1 and dsg3) is an efficient tool for confirming the diagnosis of PV. Specific antibody titers correlate with disease severity; however, desmoglein testing cannot differentiate between the various morphologic subtypes of PV.<\/jats:p>","DOI":"10.1111\/j.1365-4632.2006.02593.x","type":"journal-article","created":{"date-parts":[[2006,4,28]],"date-time":"2006-04-28T09:49:10Z","timestamp":1146217750000},"page":"518-522","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Evaluation of desmoglein enzyme\u2010linked immunosorbent assay (ELISA) in Indian patients with pemphigus vulgaris"],"prefix":"10.1111","volume":"45","author":[{"given":"Vinod K.","family":"Sharma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. R. 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