{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:39:05Z","timestamp":1761662345359},"reference-count":9,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,2,17]],"date-time":"2006-02-17T00:00:00Z","timestamp":1140134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> Cutaneous leishmaniasis (CL) has long been reported in the Cukurova region. We have compared the sensitivity of the conventional methods of diagnosis by microscopy and cultivation of lesion aspirates against polymerase chain reaction (PCR) amplification of parasite\u2010specific DNA from these samples.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> The samples (<jats:italic>n<\/jats:italic>\u00a0=\u00a025) were obtained from patients clinically diagnosed with CL at the regional dermatology clinic. Aliquots of the samples were stained with Giemsa for microscopy and cultured in Novy\u2010Nicolle\u2010McNeal (NNN) blood agar for promastigote growth. The remainder were subjected to DNA extraction for PCR amplification of the conserved region of kinetoplast minicircle DNA. PCR products of the expected size (120\u00a0bp) were observed after agarose gel electrophoresis, followed by staining with ethidium bromide.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> The positive rates from 25 samples were 44%, 68%, and 100% for cultivation, microscopy, and PCR, respectively.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> The PCR method used appears to be the most sensitive for the diagnosis of CL in this region.<\/jats:p>","DOI":"10.1111\/j.1365-4632.2006.02695.x","type":"journal-article","created":{"date-parts":[[2006,2,17]],"date-time":"2006-02-17T15:04:18Z","timestamp":1140188658000},"page":"569-572","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Comparison of conventional and polymerase chain reaction diagnostic techniques for leishmaniasis in the endemic region of Adana, Turkey"],"prefix":"10.1111","volume":"45","author":[{"given":"Gulnaz","family":"Culha","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soner","family":"Uzun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kadri","family":"Ozcan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamdi R.","family":"Memisoglu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwang\u2010Poo","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,2,17]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(90)90031-7"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"348","DOI":"10.4269\/ajtmh.1993.49.348","article-title":"Diagnosis of Leishmania using the polymerase chain reaction: a simplified procedure for field work","volume":"49","author":"Lopez M","year":"1993","journal-title":"Am J Trop Med Hyg"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(96)90112-1"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.2307\/3285616"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1999.02673.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2000.tb00088.x"},{"key":"e_1_2_6_8_2","first-page":"201","article-title":"A comparative study of diagnosis by the polymerase chain reaction and by current clinical methods using biopsies from Colombian patients with suspected leishmaniasis","volume":"44","author":"De Brujin MH","year":"1993","journal-title":"Trop Med Parasitol"},{"key":"e_1_2_6_9_2","doi-asserted-by":"crossref","first-page":"102","DOI":"10.4269\/ajtmh.1998.58.102","article-title":"Simplified PCR detection of new world Leishmania in clinical specimens of cutaneous leishmaniasis","volume":"58","author":"Belli A","year":"1998","journal-title":"Am J Trop Med Hyg"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(95)90537-5"}],"container-title":["International Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-4632.2006.02695.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-4632.2006.02695.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T02:10:05Z","timestamp":1696903805000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-4632.2006.02695.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,2,17]]},"references-count":9,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1111\/j.1365-4632.2006.02695.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-4632.2006.02695.x","archive":["Portico"],"relation":{},"ISSN":["0011-9059","1365-4632"],"issn-type":[{"value":"0011-9059","type":"print"},{"value":"1365-4632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,2,17]]},"assertion":[{"value":"2006-02-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}