{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T08:45:49Z","timestamp":1762505149561},"reference-count":11,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,19]],"date-time":"2006-07-19T00:00:00Z","timestamp":1153267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2007,3]]},"abstract":"A 74\u2010year\u2010old woman presented with painful ulcerative nodules on the left forearm. She had received systemic steroid therapy for rheumatoid arthritis for several years. On physical examination, there were four hemorrhagic ulcerative nodules with a linear distribution on the left forearm (Fig.\u00a01A<\/jats:ext-link>). These nodules had developed over the course of 2\u00a0months, and the number of lesions had increased despite systemic antibiotic therapy. There was no sign of systemic dissemination of the disease.<\/jats:p>(A) Four ulcerative hemorrhagic nodules with linear pattern on the left forearm. (B) Suppurative granulomatous infiltration composed of epithelioid histiocytes, multinucleated giant cells, and neutrophils in the dermis (hematoxylin and eosin; original magnification, \u00d7400). (C) Numerous nonbranching septate hyphae in the dermis stained positive with Gomori\u2013methenamine silver stain<\/jats:p><\/jats:caption>image<\/jats:alt-text><\/jats:graphic><\/jats:fig><\/jats:p>Biopsy of a nodule demonstrated suppurative granulomatous infiltration (Fig.\u00a01B<\/jats:ext-link>); the hyphae stained positive with periodic acid\u2013Schiff (data is not shown) and Gomori\u2013methenamine silver stains in the dermis (Fig.\u00a01C<\/jats:ext-link>). The biopsy specimen was cultured in Sabouraud's dextrose agar supplemented with cycloheximide with incubation at 26\u00a0\u00b0C. A yeast\u2010like creamy colony grew in 1\u00a0week. The colony became yellowish gray in color and the surface folded radially after 4\u00a0weeks of incubation (Fig.\u00a02A<\/jats:ext-link>). Microscopic examination revealed arthroconidia and blastoconidia (Fig.\u00a02B<\/jats:ext-link>), and urease activity was positive. The fungus was identified as Trichosporon beigelii<\/jats:italic> by yeast biochemical card (YBC, Biomerieux Vitek, Inc., Hazelwood, MO, USA). The sequences of rDNA obtained from the colony were amplified using polymerase chain reaction (PCR) primer, analyzing the sequences of the 5.8S and 28S rDNA regions for the genetic identification of the Trichosporon<\/jats:italic> species. The sequences of the PCR product matched the corresponding sequences of the T. inkin<\/jats:italic> strain with 99% accuracy (Fig.\u00a02C<\/jats:ext-link>). The patient was given oral itraconazole for 8\u00a0weeks with good clinical results.<\/jats:p>(A) Radially folded colony with yellowish gray color grew after 4\u00a0weeks of incubation (Sabouraud's dextrose agar, 26\u00a0\u00b0C). (B) Articulated arthroconidia (arrowhead) and budding blastoconidia (arrow) were seen (lactophenol cotton blue; original magnification, \u00d7\u00a0400). (C) Polymerase chain reaction (PCR) amplification products of the 337\u2010bp fragment of ribosomal DNA from our colony. Lane 1, molecular weight marker; lane 2, Trichosporon inkin<\/jats:italic> from our case<\/jats:p><\/jats:caption>image<\/jats:alt-text><\/jats:graphic><\/jats:fig><\/jats:p>","DOI":"10.1111\/j.1365-4632.2006.03087.x","type":"journal-article","created":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T15:50:51Z","timestamp":1153410651000},"page":"282-283","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Trichosporon inkin<\/i> subcutaneous infection in a rheumatoid arthritis patient"],"prefix":"10.1111","volume":"46","author":[{"given":"Hyun Jeong","family":"Song","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Lip","family":"Chung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyu Suk","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,3,2]]},"reference":[{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.1994.tb00277.x"},{"key":"e_1_2_2_3_2","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0891-5520(20)30245-2","article-title":"Trichosporonosis","volume":"3","author":"Walsh TJ","year":"1989","journal-title":"Infect Dis Clin North Am"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-4725.1989.tb03250.x"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.07.066"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200010000-00023"},{"key":"e_1_2_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(01)00215-2"},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.5.2341-2344.2004"},{"key":"e_1_2_2_9_2","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1093\/clinids\/23.2.396","article-title":"Trichosporon inkin endocarditis: short\u2010term evolution and clinical report","volume":"23","author":"Chaumentin G","year":"1996","journal-title":"Clin Infect Dis"},{"key":"e_1_2_2_10_2","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1023\/A:1006870017725","article-title":"Trichosporon inkin peritonitis during continuous ambulatory peritoneal dialysis with bibliography review","volume":"139","author":"Lopes JO","year":"1997","journal-title":"Mycopathologica"},{"key":"e_1_2_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.12.5827-5829.2003"},{"key":"e_1_2_2_12_2","doi-asserted-by":"publisher","DOI":"10.5414\/CNP59069"}],"container-title":["International Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-4632.2006.03087.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-4632.2006.03087.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T09:16:20Z","timestamp":1697361380000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-4632.2006.03087.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3]]},"references-count":11,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,3]]}},"alternative-id":["10.1111\/j.1365-4632.2006.03087.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-4632.2006.03087.x","archive":["Portico"],"relation":{},"ISSN":["0011-9059","1365-4632"],"issn-type":[{"value":"0011-9059","type":"print"},{"value":"1365-4632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,3]]},"assertion":[{"value":"2007-03-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}