{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T06:47:18Z","timestamp":1773211638591,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,4,30]],"date-time":"2007-04-30T00:00:00Z","timestamp":1177891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> The histopathologic changes characteristic of psoriasis might be related to suppressed apoptosis. The P53 and Bcl\u20102 proteins play a central role in the regulation of apoptosis. This study aimed to evaluate P53 and Bcl\u20102 expression and apoptotic cells in the psoriatic skin before and after topical calcipotriol therapy.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> Skin biopsies were obtained from nonlesional and lesional skin of 10\u00a0patients with generalized plaque psoriasis before and after treatment with topical calcipotriol ointment. The P53 and Bcl\u20102 expression was evaluated using immunoperoxidase technique and apoptotic cells by the terminal deoxynucleotide transferase (TdT) mediated deoxyuridine triphosphate nick end labeling (TUNEL) method.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> After topical calcipotriol therapy, keratinocytes of psoriatic skin showed significant decrease of P53 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.002) and increase of Bcl\u20102 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.01) expression. On the other hand lymphocytes showed significant decrease of Bcl\u20102 (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.01). There were no apoptotic cells before treatment but after calcipotriol therapy, apoptosis was more detectable in keratinocytes than in lymphocytes.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> The results of the study suggested that one of the actions of calcipotriol in psoriasis might be exerted through induction of apoptosis, especially of keratinocytes, through a P53\u2010independent pathway. Meanwhile, suppression of Bcl\u20102 expression in lymphocytes may promote apoptosis of dermal lymphocytes leading to healing of psoriasis.<\/jats:p>","DOI":"10.1111\/j.1365-4632.2007.03099.x","type":"journal-article","created":{"date-parts":[[2007,5,7]],"date-time":"2007-05-07T15:26:36Z","timestamp":1178551596000},"page":"468-474","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Apoptosis, P53 and Bcl\u20102 expression in response to topical calcipotriol therapy for psoriasis"],"prefix":"10.1111","volume":"46","author":[{"given":"Moetaz","family":"El\u2010Domyati","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manal","family":"Barakat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasha","family":"Abdel\u2010Razek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tag","family":"El\u2010Din Anbar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,4,30]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(03)01134-4"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1159\/000018394"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(97)80266-6"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7878464"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(01)00162-1"},{"key":"e_1_2_6_7_2","first-page":"538","article-title":"Over\u2010expression of Bcl\u20102 in lymphocytes of psoriatic skin","volume":"17","author":"Yildiz L","year":"2003","journal-title":"JEADV"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/348334a0"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/351453a0"},{"key":"e_1_2_6_10_2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1001\/archderm.1994.01690020091015","article-title":"Abnormalities of P53 expression in cutaneous disorders","volume":"130","author":"McNutt NS","year":"1994","journal-title":"Arch Dermatol"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004280050327"},{"key":"e_1_2_6_12_2","first-page":"55","article-title":"Treatment of psoriasis vulgaris with topical calcipotriol: is the clinical improvement of lesional skin related to a down\u2010regulation of cell adhesion molecules?","volume":"186","author":"Cagnoni ML","year":"1994","journal-title":"Acta Derm Venereol"},{"key":"e_1_2_6_13_2","first-page":"171","article-title":"Topical calcipotriol for psoriasis \u2013 an immunohistologic study","volume":"186","author":"Mozzanica N","year":"1994","journal-title":"Acta Derm Venereol"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s000110050042"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1747.2003.12626.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1159\/000250839"},{"key":"e_1_2_6_17_2","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1001\/archderm.1997.03890430031005","article-title":"Apoptotic and proliferating cells in cutaneous lymphoproliferative diseases","volume":"133","author":"Kikuchi A","year":"1997","journal-title":"Arch Dermatol"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.2174\/1568010043343930"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000372-197900130-00002"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1994.tb08552.x"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1080\/00015550310007427"},{"key":"e_1_2_6_22_2","first-page":"433","article-title":"Effect of topical tretinoin, chemical peeling and dermabrasion on P53 expression in facial skin","volume":"13","author":"El\u2010Domyati MM","year":"2003","journal-title":"Eur J Dermatol"},{"key":"e_1_2_6_23_2","first-page":"33","article-title":"P53 and oncogenes expression in psoriasis","volume":"146","author":"Tadini G","year":"1989","journal-title":"Acta Derm Venereol"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1080\/000155599750010959"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19940915)74:6<1720::AID-CNCR2820740613>3.0.CO;2-T"},{"key":"e_1_2_6_26_2","first-page":"1321","article-title":"Keratinocytes derived from psoriatic plaques are resistant to apoptosis compared with normal skin","volume":"15","author":"Wrone\u2010Smith T","year":"1997","journal-title":"Am J Pathol"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711690408"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1997.tb01105.x"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.02-1198"},{"key":"e_1_2_6_30_2","first-page":"2304","article-title":"Apoptosis is induced by the active metabolite of vitamin D3 and its analogue EB1089 in colorectal adenoma and carcinoma cells: possible implications for prevention and therapy","volume":"60","author":"Diaz GD","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_6_31_2","first-page":"413","article-title":"Apoptosis and the skin","volume":"9","author":"Teraki Y","year":"1999","journal-title":"Eur J Dermatol"}],"container-title":["International Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-4632.2007.03099.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-4632.2007.03099.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T22:39:51Z","timestamp":1696891191000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-4632.2007.03099.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4,30]]},"references-count":30,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,5]]}},"alternative-id":["10.1111\/j.1365-4632.2007.03099.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-4632.2007.03099.x","archive":["Portico"],"relation":{},"ISSN":["0011-9059","1365-4632"],"issn-type":[{"value":"0011-9059","type":"print"},{"value":"1365-4632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4,30]]},"assertion":[{"value":"2007-04-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}