{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T10:51:02Z","timestamp":1753354262167},"reference-count":8,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2009,11,17]],"date-time":"2009-11-17T00:00:00Z","timestamp":1258416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background\u2002<\/jats:bold> An 81\u2010year\u2010old man presented with a skin ulcer on the left forearm caused by infection with <jats:italic>Mycobacterium ulcerans <\/jats:italic>ssp.<jats:italic> shinshuense<\/jats:italic>. The patient first noticed the subcutaneous nodule with an undermined ulcer and areola on the left forearm without any episode of trauma.<\/jats:p><jats:p><jats:bold>Methods\u2002<\/jats:bold> The rod\u2010shaped and acid\u2010fast bacteria taken from the ulcerative lesion were positive for Ziehl\u2013Neelsen staining. The bacterial colony was cultured on Ogawa slant egg medium at 28\u00a0\u00b0C.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> A clinical diagnosis of <jats:italic>Mycobacterium<\/jats:italic> infection was made. For therapy, in addition to oral clarithromycin, topical sulfadiazine silver and hyperthermia were used. One month after starting treatment, topical treatment was changed to U\u2010pasta (sucrose, povidone\u2010iodine). Four months after the onset of the disease, bacterial colonies composed of scotochromogens were identified as <jats:italic>Mycobacterium marinum<\/jats:italic> by the DNA\u2013DNA hybridization (DDH) method. The growth speed and characteristics of the bacterial colonies were different from those of <jats:italic>Mycobacterium marinum<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> This pathogenetic bacterium was finally identified as <jats:italic>Mycobacterium ulcerans <\/jats:italic>ssp. <jats:italic>shinshuense<\/jats:italic> by the polymerase chain reaction method and 16S rRNA gene sequencing. Nine months after the onset of the disease, the ulcer was re\u2010epithelialized with a residual scar.<\/jats:p>","DOI":"10.1111\/j.1365-4632.2008.04060.x","type":"journal-article","created":{"date-parts":[[2009,11,17]],"date-time":"2009-11-17T10:36:59Z","timestamp":1258454219000},"page":"1330-1333","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Leg ulcer caused by <i>Mycobacterium ulcerans<\/i> ssp. <i>shinshuense<\/i> infection"],"prefix":"10.1111","volume":"48","author":[{"given":"Makoto","family":"Kondo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichiro","family":"Kurokawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiyuki","family":"Ito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kei\u2010ichi","family":"Yamanaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshio","family":"Yamazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Mizutani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,11,17]]},"reference":[{"key":"e_1_2_4_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1982.tb00241.x"},{"key":"e_1_2_4_3_2","first-page":"437","article-title":"Mycobacterium shinshuense isolated from cutaneous ulcer lesion of right lower extremity in 37\u2010year\u2010old woman","volume":"79","author":"Kazumi Y","year":"2004","journal-title":"Kekkaku"},{"key":"e_1_2_4_4_2","first-page":"271","article-title":"Therapy resistant ulcer on the elbow due to Mycobacterium shinshuense  infection","volume":"24","author":"Imada H","year":"2007","journal-title":"J Jpn Soc Surg Hand"},{"key":"e_1_2_4_5_2","first-page":"842","article-title":"A case of cutaneous Mycobacterium shinshuense infection","volume":"116","author":"Taki R","year":"2006","journal-title":"Jpn J Dermatol"},{"key":"e_1_2_4_6_2","first-page":"145","article-title":"Skin ulcer due to Mycobacterium ulcerans subsp. shinshuense","volume":"30","author":"Suzuki S","year":"2007","journal-title":"Hifubyo-Shinryo"},{"key":"e_1_2_4_7_2","first-page":"829","article-title":"Skin infection due to Mycobacterium shinshuense","volume":"118","author":"Funakoshi K","year":"2008","journal-title":"Jpn J Dermatol"},{"key":"e_1_2_4_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01041-07"},{"key":"e_1_2_4_9_2","doi-asserted-by":"publisher","DOI":"10.1159\/000246041"}],"container-title":["International Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-4632.2008.04060.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-4632.2008.04060.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T07:27:08Z","timestamp":1697354828000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-4632.2008.04060.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11,17]]},"references-count":8,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2009,12]]}},"alternative-id":["10.1111\/j.1365-4632.2008.04060.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-4632.2008.04060.x","archive":["Portico"],"relation":{},"ISSN":["0011-9059","1365-4632"],"issn-type":[{"value":"0011-9059","type":"print"},{"value":"1365-4632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,11,17]]},"assertion":[{"value":"2009-11-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}