{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:56:13Z","timestamp":1787014573329,"version":"build-2736575974"},"reference-count":8,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2010,9,28]],"date-time":"2010-09-28T00:00:00Z","timestamp":1285632000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Dermatology"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Background\u2002<\/jats:bold>\n                    Progressive macular hypomelanosis (PMH), a condition of uncertain etiology, is characterized by asymptomatic hypopigmented macules, predominantly located on the trunk. To date, the study of this disease has been sporadic and there are still no clinical diagnostic criteria. The aim of this study was to investigate the histopathologic and ultrastructural characteristics of PMH, and propose the clinical diagnostic criteria of PMH.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods\u2002<\/jats:bold>\n                    The Wood\u2019s lamp and Confocal Laser Scanning Microscopy were used to observe the lesions\u2019 features. Skinbiopsies were used for hematoxylin and eosin staining, melanin staining, antibodies staining of S\u2010100 protein, tyrosinase\u2010related protein\u20101(TRP\u20101) and tyrosinase (T311), and also for ultra\u2010structural study. Melanocytes were isolated and cultured from the lesions.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results\u2002<\/jats:bold>\n                    Under Wood\u2019s lamp examination, the lesions of PMH showed punctiform red fluorescence. Confocal Laser Scanning Microscopy observation of the lesion showed that its \u201cpigmented ring\u201d around the dermal papillae was intact, but its melanin content was decreased compared with the surrounding normal skin. Ferrous sulfate staining showed that melanin content in the lesion of PMH was significantly decreased compared with the normal skin (\n                    <jats:italic>P<\/jats:italic>\n                    \u2003&lt;\u20030.05). S\u2010100 staining showed that the number of positive cells in the basal layer had no statistical significance (\n                    <jats:italic>P<\/jats:italic>\n                    \u2003&gt;\u20030.05) between the lesion areas (8.25\u2003\u00b1\u20030.96) and the surrounding normal skin (8.75\u2003\u00b1\u20031.71). TRP\u20101 staining showed no significant difference between lesion areas (4.25\u2003\u00b1\u20030.96) and the surrounding normal skin (4.50\u2003\u00b1\u20031.29) (\n                    <jats:italic>P<\/jats:italic>\n                    \u2003&gt;\u20030.05), and T311 staining also showed no difference between lesion areas (4.01\u2003\u00b1\u20030.87) and the surrounding normal skin (4.30\u2003\u00b1\u20031.05) (\n                    <jats:italic>P<\/jats:italic>\n                    \u2003&gt;\u20030.05). Ultra\u2010structural studies revealed a large reduction in the number of mature melanosome from PMH lesions. There were many membrane\u2010bound groups in PMH lesions with normal appearance the margin, which contained a number of smaller type II\u2013IV melanosomes, which were distributed in clusters. No degradation of melanosomes was present in the lysosomal compartments of PMH lesions. When melanocytes from the PMH lesions were cultured\n                    <jats:italic>in vitro<\/jats:italic>\n                    , the morphology of those melanocytes showed no difference compared with normal melanocytes.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion\u2002<\/jats:bold>\n                    As a result of the above findings, we discussed and summarized the PMH\u2019s clinical diagnostic criteria.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-4632.2010.04492.x","type":"journal-article","created":{"date-parts":[[2010,9,28]],"date-time":"2010-09-28T12:07:24Z","timestamp":1285675644000},"page":"1127-1132","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Clinical, pathologic, and ultrastructural studies of progressive macular hypomelanosis"],"prefix":"10.1111","volume":"49","author":[{"given":"Xin\u2010gang","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai\u2010e","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiu\u2010zu","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun\u2010hui","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,9,28]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.1988.tb00561.x"},{"key":"e_1_2_5_3_2","first-page":"197","article-title":"Effects of fructus ligustri lucidi on the tyrosinase activity and melanogenesis of cultrued human melanocytes","volume":"39","author":"Zhang DM","year":"2006","journal-title":"Zhong Hua Pi Fu Ke Za Zhi"},{"key":"e_1_2_5_4_2","first-page":"317","article-title":"Cutis trunci variata: nueva genodermatosis","volume":"15","author":"Borelli D","year":"1987","journal-title":"Med Cutanea Ibero Lat Am"},{"key":"e_1_2_5_5_2","first-page":"416","volume-title":"Yearbook of Dermatology","author":"Fitzpatrick TB","year":"1996"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.140.2.210"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2006.02755.x"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/00022744-200209000-00002"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2007.02515.x"}],"container-title":["International Journal of Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-4632.2010.04492.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-4632.2010.04492.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T12:12:41Z","timestamp":1698667961000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-4632.2010.04492.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,28]]},"references-count":8,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2010,10]]}},"alternative-id":["10.1111\/j.1365-4632.2010.04492.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-4632.2010.04492.x","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.6363956.6462054","asserted-by":"object"}]},"ISSN":["0011-9059","1365-4632"],"issn-type":[{"value":"0011-9059","type":"print"},{"value":"1365-4632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9,28]]},"assertion":[{"value":"2010-09-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}