{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T03:42:15Z","timestamp":1770349335136,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2004,6,4]],"date-time":"2004-06-04T00:00:00Z","timestamp":1086307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> We characterized by immunoblotting the antigenicity of the most frequent fungi colonizing cork during its industrial processing, <jats:italic>Penicillium glabrum<\/jats:italic> and <jats:italic>Chrysonilia sitophila<\/jats:italic>. <jats:italic>Penicillium glabrum<\/jats:italic> is the main causative agent of Suberosis, a hypersensitivity pneumonitis of cork workers. <jats:italic>Chrysonilia sitophila<\/jats:italic> induces both IgE sensitization and occupational asthma in the wood processing industry.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Serum\u2010specific IgG, IgG4 and IgE to <jats:italic>P. glabrum<\/jats:italic> and <jats:italic>C. sitophila<\/jats:italic> from nine cork workers with hypersensitivity pneumonitis (HP) and seven with asthma (four with occupational asthma) were analysed by immunoblotting.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Both HP and asthmatic patients\u2019 sera showed immunoreactivity to several proteins resolved in the specific immunoblot strips. The frequency of specific IgG recognition to 12\u201313.5 and 33\u2003kDa proteins of <jats:italic>P. glabrum<\/jats:italic> was significantly higher in HP patients. The sera of HP patients had significantly higher specific IgG recognition to 16 and 51\u201355\u2003kDa proteins of <jats:italic>C. sitophila<\/jats:italic>. There was no specific IgE recognition in the sera of HP or asthmatic patients to both fungi.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Different patterns of antibody reactivity to <jats:italic>P. glabrum<\/jats:italic> and <jats:italic>C. sitophila<\/jats:italic> are seen in cork workers with hypersensitivity pneumonitis or asthma. The 12\u201313.5 and 33\u2003kDa proteins of <jats:italic>P. glabrum<\/jats:italic> and the 16 and 51\u201355\u2003kDa proteins of <jats:italic>C. sitophila<\/jats:italic> may be major antigens in Suberosis.<\/jats:p>","DOI":"10.1111\/j.1398-9995.2004.00472.x","type":"journal-article","created":{"date-parts":[[2004,6,4]],"date-time":"2004-06-04T08:26:32Z","timestamp":1086337592000},"page":"739-745","source":"Crossref","is-referenced-by-count":22,"title":["Antigen characterization of major cork moulds in Suberosis (cork worker's pneumonitis) by immunoblotting"],"prefix":"10.1111","volume":"59","author":[{"given":"J. C.","family":"Winck","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Delgado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Murta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Lopez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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