{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T12:31:40Z","timestamp":1762605100996},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2004,6,4]],"date-time":"2004-06-04T00:00:00Z","timestamp":1086307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Multiple immunoglobulin E (IgE)\u2010binding proteins in natural rubber latex extracts have been identified. In the case of Hev b 6 a differentiation was made between the precursor protein prohevein (Hev b 6.01) and its two post\u2010transcriptionally formed proteins, the N\u2010terminal hevein (Hev b 6.02) and the C\u2010terminal domain (Hev b 6.03). All three components act as independent allergens. The aim of this study was a detailed analysis of the T\u2010cell responses and the IgE\u2010binding capacity of Hev b 6.01, Hev b 6.02 and Hev b 6.03 by using these allergens as recombinant maltose\u2010binding fusion (MBP) proteins and the usage of synthetic modified hevein peptides.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Latex\u2010allergic health care workers (HCWs) suffering from rhinitis, conjunctivitis, contact urticaria and\/or asthma with increased specific IgE\u2010antibodies to latex were tested for their IgE\u2010binding capacity and T\u2010cell reactivity (by proliferation response) to the recombinant MBP\u2010rHev b 6.01, MBP\u2010rHev b 6.02, MBP\u2010rHev b 6.03, to native Hev b 6.02, to modified hevein peptides and wheat germ agglutinin (WGA). For testing of the human leucocyte antigen (HLA) class II restriction of MBP\u2010rHev b 6.01 induced peripheral blood mononuclear cell (PBMC) responses, monoclonal antibodies against HLA\u2010DR, HLA\u2010DP or HLA\u2010DQ were added.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Seventeen of 18 (94%) serum samples from latex\u2010allergic HCWs had increased levels of specific IgE to MBP\u2010rHev b 6.01, 16 (89%) to MBP\u2010rHev b 6.02 and 13 (72%) to MBP\u2010rHev b 6.03. A significant difference existed between the specific IgE\u2010values of MBP\u2010rHev b 6.02 and MBP\u2010rHev b 6.03 (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01). Proliferation responses of PBMC of the same 18 latex\u2010allergic patients were positive for MBP\u2010rHev b 6.01 and MBP\u2010rHev b 6.03 in 83 and 67% of the tested PBMC suspension, whereas the proliferation responses induced with MBP\u2010rHev b 6.02 or native Hev b 6.02 were very low (5.6 and 22.2%). Sera from nine additional latex\u2010allergic patients showed specific IgE binding to the native Hev b 6.02, but none of these sera showed specific IgE binding to the modified Hev b 6.02\u2010peptides [whereby all eight cysteine residues were substituted by serine (C\u2003\u2192\u2003S) or by alanine (C\u2003\u2192\u2003A)]. Proliferation responses induced by the modified Hev b 6.02 peptides were not significantly different from that induced by Hev b 6.02. Potential HLA\u2010DR4Dw4(DRB1*0401)\u2010restricted T\u2010cell epitopes of Hev b 6.01 predicted by two computer algorithms were only found in the Hev b 6.03\u2010part of Hev b 6.01.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> In the Hev b 6.01 precursor the regions responsible for IgE binding and those for inducing the T\u2010cell proliferation responses are settled in different parts of the protein. The Hev b 6.02 domain is responsible for IgE binding and carries discontinuous B\u2010cell epitopes whereas Hev b 6.03 is a better inducer of a proliferation response and contains HLA\u2010DR4\u2010binding motifs.<\/jats:p>","DOI":"10.1111\/j.1398-9995.2004.00475.x","type":"journal-article","created":{"date-parts":[[2004,6,4]],"date-time":"2004-06-04T08:26:32Z","timestamp":1086337592000},"page":"724-733","source":"Crossref","is-referenced-by-count":15,"title":["Characterization of B\u2010 and T\u2010cell responses and HLA\u2010DR4 binding motifs of the latex allergen Hev b 6.01 (prohevein) and its post\u2010transcriptionally formed proteins Hev b 6.02 and Hev b 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