{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T06:26:14Z","timestamp":1788330374751,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> No strawberry allergen has so far been identified and characterized.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Serum samples were collected from patients with a suggestive case history of adverse reactions to strawberry and other fruits. Extracts from fresh and frozen strawberries were analysed by sodium dodecyl sulphate\u2010polyacrylamide gel electrophoresis (SDS\u2010PAGE), Western blotting and mass spectrometry. Patient blood samples were analysed for inhibition of IgE binding and basophil degranulation.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Several IgE\u2010binding proteins could be detected. In more than half of the patient sera, a 20\/18\u2010kDa doublet band was observed in Western blotting. These two bands were excised and analysed by mass spectrometry showing the presence of proteins belonging to the Bet v 1 family of allergens. Inhibition of the IgE binding to the 20\/18\u2010kDa doublet was obtained by addition of two recombinantly expressed allergens belonging to the Bet v 1 family (Bet v 1 and Mal d 1) and strawberry protein extract. In a cell\u2010based assay of patient blood samples, basophil degranulation could be induced by strawberry protein extract and by Bet v 1 and Mal d 1.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> We conclude that strawberry homologues to Bet v 1 may be allergens of importance for adverse reactions to strawberry.<\/jats:p>","DOI":"10.1111\/j.1398-9995.2004.00585.x","type":"journal-article","created":{"date-parts":[[2004,10,26]],"date-time":"2004-10-26T12:03:42Z","timestamp":1098792222000},"page":"1277-1284","source":"Crossref","is-referenced-by-count":46,"title":["Bet v 1 homologues in strawberry identified as IgE\u2010binding proteins and presumptive allergens"],"prefix":"10.1111","volume":"59","author":[{"given":"A. 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