{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:41:47Z","timestamp":1790710907683,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,11,2]],"date-time":"2004-11-02T00:00:00Z","timestamp":1099353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Desloratadine is a selective H<jats:sub>1<\/jats:sub>\u2010antihistamine used in the treatment of allergic rhinitis and chronic idiopathic urticaria. Desloratadine inhibits the release of allergic inflammatory mediators <jats:italic>in vitro<\/jats:italic>. We studied the impact of desloratadine on mast cell degranulation due to activation and re\u2010activation by the secretagogue, compound 48\/80.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Rat peritoneal eluate containing 5\u20136% mast cells were activated by a low concentration of compound 48\/80 in a medium containing the vital fluorescent dye, Sulforhodamine\u2010B (SFRM\u2010B, 200\u2003<jats:italic>\u03bc<\/jats:italic>g\/ml), which is engulfed by activated mast cells. The fluorescent image of activated mast cells was captured digitally and the total fluorescent area was analyzed when desloratadine was applied before or after compound 48\/80.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Mast cells were not activated by desloratadine (10<jats:sup>\u22124<\/jats:sup>\u2003M), SFRM\u2010B (200\u2003<jats:italic>\u03bc<\/jats:italic>g\/ml), or diluent alone. A low concentration of compound 48\/80 (0.125\u2003<jats:italic>\u03bc<\/jats:italic>g\/ml) induced fluorescence, while mast cells lost fluorescent images due to further degranulation on re\u2010exposure to compound 48\/80. Desloratadine (10<jats:sup>\u22128<\/jats:sup>\u201310<jats:sup>\u22124<\/jats:sup>\u2003M), inhibited compound 48\/80\u2010induced mast cell degranulation in a concentration\u2010dependent manner. Desloratadine also reduced the loss of fluorescent images due to re\u2010exposure to compound 48\/80.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Desloratadine may have a mast cell stabilizing effect at low concentrations in response to repeated mast cell activation <jats:italic>in vitro<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1398-9995.2004.00641.x","type":"journal-article","created":{"date-parts":[[2004,12,3]],"date-time":"2004-12-03T09:27:17Z","timestamp":1102066037000},"page":"117-124","source":"Crossref","is-referenced-by-count":22,"title":["Desloratadine prevents compound 48\/80\u2010induced mast cell degranulation: visualization using a vital fluorescent dye technique"],"prefix":"10.1111","volume":"60","author":[{"given":"Y. H.","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Tach\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. G.","family":"Harris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Kreutner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. F.","family":"Daly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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