{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T10:31:33Z","timestamp":1774866693325,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,12,16]],"date-time":"2004-12-16T00:00:00Z","timestamp":1103155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Wheat can cause severe immunoglobulin E (IgE)\u2010mediated systemic reactions including anaphylaxis but knowledge on relevant wheat allergens at the molecular level is scanty.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Seven children (aged from 6\u2003months to 13\u2003years) experiencing from 2 to 10 anaphylactic reactions in a year after eating food\u2010containing wheat were examined. Purified <jats:italic>\u03c9<\/jats:italic>\u20105 gliadin was used as an allergen in IgE enzyme\u2010linked immunosorbent assay (ELISA) and in skin prick testing (SPT). Wheat CAP radioallergosorbent test (RAST) and SPT were also examined.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> All seven anaphylactic children, but none of 15 control subjects had IgE antibodies to <jats:italic>\u03c9<\/jats:italic>\u20105 gliadin in ELISA. Five of the six tested anaphylactic children showed positive SPT to <jats:italic>\u03c9<\/jats:italic>\u20105 and crude gliadin, and all seven had positive wheat CAP RAST and SPT. One child was challenged with wheat, which caused anaphylaxis. After adherence to a wheat\u2010free diet four children remained symptomless and three experienced one to two anaphylactic reactions.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The present results show that wheat <jats:italic>\u03c9<\/jats:italic>\u20105 gliadin is a major sensitizing allergen in children with wheat\u2010induced anaphylaxis. They also suggest that <jats:italic>\u03c9<\/jats:italic>\u20105 gliadin IgE ELISA could be used as a diagnostic test for this severe allergy.<\/jats:p>","DOI":"10.1111\/j.1398-9995.2004.00656.x","type":"journal-article","created":{"date-parts":[[2005,2,23]],"date-time":"2005-02-23T12:20:18Z","timestamp":1109161218000},"page":"506-509","source":"Crossref","is-referenced-by-count":36,"title":["IgE antibodies to <i>\u03c9<\/i>\u20105 gliadin in children with wheat\u2010induced 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