{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:54:04Z","timestamp":1790243644884,"version":"4.1.0"},"reference-count":12,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,4,6]],"date-time":"2005-04-06T00:00:00Z","timestamp":1112745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> A temporarily elevated level of serum mast cell tryptase (ST) indicates mast cell activation and occurs in systemic anaphylactic reactions (SAR). We measured ST following a sting challenge in vespid venom\u2010allergic patients treated with venom immunotherapy (VIT) and in healthy controls, respectively.<\/jats:p><jats:p><jats:bold>Aim of the study:\u2002<\/jats:bold> To assess changes of ST over time in vespid venom\u2010allergic patients at the occasion of a re\u2010sting and in healthy controls.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> A sting challenge was performed in 20 patients on vespid VIT to monitor efficacy of VIT. ST was measured between 9.00 and 10.00\u2003a.m. (baseline). Sting challenge was performed at 2.00\u2003p.m., and ST was determined again 20\u2003min, 90\u2003min and 18\u2003h later. Measurements at corresponding times of the day were done in nine healthy controls.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> One patient developed a mild SAR to the sting challenge which was associated with a temporary increase of ST. In the other 19 patients who tolerated the sting challenge without SAR ST decreased significantly by 18.0% (median, range 8.3\u201336.7%). Twenty minutes after the sting when compared with baseline levels (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001), a significant decrease of ST was still present after 90\u2003min (median 13.7%) (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001), but not after 18\u2003h (<jats:italic>P<\/jats:italic>\u2003=\u20030.57). A comparably significant temporary decline was found in controls.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> The temporary decline of ST in patients and in controls suggests a circadian variation of ST concentration. A normal diurnal pattern of ST concentration after sting challenge is associated with successful treatment.<\/jats:p>","DOI":"10.1111\/j.1398-9995.2005.00771.x","type":"journal-article","created":{"date-parts":[[2005,4,6]],"date-time":"2005-04-06T15:05:26Z","timestamp":1112799926000},"page":"689-692","source":"Crossref","is-referenced-by-count":22,"title":["Possible circadian variation of serum mast cell tryptase concentration"],"prefix":"10.1111","volume":"60","author":[{"given":"S.","family":"Dugas\u2010Breit","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Przybilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Sch\u00f6pf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Ru\u00ebff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,4,6]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.138.7.2184"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2003.00253.x"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198706253162603"},{"key":"e_1_2_5_5_2","first-page":"11","article-title":"Insect\u2010sting challenge in 138 patients: relation between clinical severity of anaphylaxis and mast cell activation","volume":"100","author":"Linden PW","year":"1992","journal-title":"J Allergy Clin Immunol"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1993.tb04755.x"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1996.tb04596.x"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.119154"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1995.tb00007.x"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(01)00038-8"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01533368"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2001.056006568.x"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1399-6576.2003.00237.x"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2005.00771.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2005.00771.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T17:58:59Z","timestamp":1697392739000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2005.00771.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,4,6]]},"references-count":12,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2005,5]]}},"alternative-id":["10.1111\/j.1398-9995.2005.00771.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2005.00771.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,4,6]]}}}