{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:53:15Z","timestamp":1792666395813,"version":"4.5.0"},"reference-count":13,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,1,23]],"date-time":"2006-01-23T00:00:00Z","timestamp":1137974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Monitoring of the allergen sensitivity of a patient is most important for optimal patient care and a basic prerequisite for immunomodulating treatment. The objective of this study was to investigate how basophil allergen sensitivity can be applied in the monitoring of anti\u2010immunoglobulin E (IgE) treatment.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Basophils from timothy grass pollen allergic patients were, by flow cytometry, analysed for allergen threshold sensitivity (CD\u2010sens) by measuring CD63 up\u2010regulation on CD203c\u2010identified basophils. The results were compared with maximal percentage CD63 up\u2010regulation at one allergen dose (CD\u2010max), skin prick test end\u2010point allergen titration, (SPT\u2010sens), nasal provocation titration tests (nasal provocation titre) and serum IgE and IgE antibody concentrations.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> There was a significant correlation (<jats:italic>r<\/jats:italic>\u2003=\u20030.50, <jats:italic>P<\/jats:italic>\u2003=\u20030.01) between CD\u2010sens and SPT\u2010sens, CD\u2010sens and the IgE antibody concentration in percentage of \u2018total IgE\u2019 (relative IgE antibody concentration) (<jats:italic>r<\/jats:italic>\u2003=\u20030.72, <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001) as well as between CD\u2010sens and nasal provocation titre (<jats:italic>r<\/jats:italic>\u2003=\u20030.54, <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05) but, in contrast, CD\u2010max did not correlate with any of the sensitization parameters, i.e. SPT\u2010sens, nasal provocation titre, absolute and relative IgE antibody concentration or CD\u2010sens. CD\u2010sens could be used to monitor omalizumab treatment efficacy while, based on CD\u2010max, four of seven symptom\u2010free patients on omalizumab would have been classified as having ongoing allergy.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> CD\u2010sens seems to be very useful for the determination of a patient's allergen sensitivity and should be evaluated for the measurement and monitoring of anti\u2010IgE treatment efficacy. CD\u2010max, the conventional approach to basophil allergen challenge, which mirrors cell reactivity, gives incorrect information.<\/jats:p>","DOI":"10.1111\/j.1398-9995.2006.00987.x","type":"journal-article","created":{"date-parts":[[2006,1,23]],"date-time":"2006-01-23T18:07:38Z","timestamp":1138039658000},"page":"298-302","source":"Crossref","is-referenced-by-count":103,"title":["Basophil allergen threshold sensitivity: a useful approach to anti\u2010IgE treatment efficacy evaluation"],"prefix":"10.1111","volume":"61","author":[{"given":"A.","family":"Nopp","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. G. O.","family":"Johansson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ankerst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Bylin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. O.","family":"Cardell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Gr\u00f6nneberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Irander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Palmqvist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"\u00d6man","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,1,23]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1989.tb04196.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1016\/S0140-6736(67)90615-0","article-title":"Diagnosis of allergy by an in vitro test for allergen antibodies","volume":"25","author":"Wide L","year":"1967","journal-title":"Lancet"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.114708"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2005.00870.x"},{"key":"e_1_2_6_6_2","first-page":"2343","article-title":"The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors","volume":"94","author":"B\u00fchring H\u2010J","year":"1999","journal-title":"Blood"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2003.11.044"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1201\/9780203909003"},{"key":"e_1_2_6_9_2","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.4049\/jimmunol.158.3.1438","article-title":"Down\u2010regulation of Fc\u025bRI expression in human basophils during in vivo treatment of atopic patients with anti\u2010IgE antibody","volume":"158","author":"MacGlashan DW","year":"1997","journal-title":"J Immunol"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.286.23.2956"},{"key":"e_1_2_6_11_2","first-page":"473","article-title":"The basophil degranulation test. A review of the literature","volume":"253","author":"Kirshbaum BA","year":"1967","journal-title":"Am J Med Sci"},{"key":"e_1_2_6_12_2","first-page":"151","article-title":"Immunoglobulin E, a new class of human immunoglobulin","volume":"38","author":"Bennich HH","year":"1968","journal-title":"Bull World Health Organ"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(91)90094-5"},{"key":"e_1_2_6_14_2","first-page":"143","article-title":"Flow cytometric basophil activation test: a review","volume":"12","author":"Sanz ML","year":"2002","journal-title":"J Investig Allergol Clin Immunol"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2006.00987.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2006.00987.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T18:49:57Z","timestamp":1696963797000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2006.00987.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,1,23]]},"references-count":13,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["10.1111\/j.1398-9995.2006.00987.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2006.00987.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,1,23]]}}}