{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T04:47:38Z","timestamp":1772772458995,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,30]],"date-time":"2005-11-30T00:00:00Z","timestamp":1133308800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Legumes are important causative agents of type I hypersensitivity in south Asia and Europe but such studies are lacking in Indian population. The present study investigates blackgram sensitization in asthma and rhinitis patients and identifies immunoglobulin E (IgE)\u2010binding proteins.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Respiratory allergy patients were evaluated using standard questionnaire and skin prick tests (SPT) with common foods and aeroallergens. Blackgram\u2010specific IgE level was estimated by enzyme\u2010linked immunosorbent assay (ELISA) and sensitization was established by a double\u2010blind, placebo\u2010controlled food challenge (DBPCFC). The cross\u2010reactivity of blackgram with other legumes was studied by immunobiochemical methods.<\/jats:p><jats:p><jats:bold>Result:\u2002<\/jats:bold> Of 816 patients, 35 gave history of blackgram hypersensitivity. From these, 16 patients were SPT positive and 14 showed elevated specific IgE (three times of negative control) to blackgram. DBPCFC established blackgram sensitivity in four of 14 patients. Immunoblotting with individual patient's sera recognized eight most prevalent allergens of 78, 56, 47, 43, 40, 30, 28 and 16\u2003kDa. Roasted blackgram showed six major allergens whereas 47, 43 and 28\u2003kDa proteins retained IgE reactivity upon boiling. Blackgram extract required 14\u2003ng of self protein for 50% ELISA inhibition whereas roasted and boiled blackgram required 16 and 120\u2003ng protein. ELISA and immunoblot inhibition show partial inhibition to blackgram proteins by lentil, limabean and pea.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Blackgram induces IgE\u2010mediated reactions in 1.7% of asthma and rhinitis patients and contains eight major IgE\u2010binding components, of which six retained IgE reactivity after roasting. Blackgram shares allergenicity with lentil and limabean.<\/jats:p>","DOI":"10.1111\/j.1398-9995.2006.00990.x","type":"journal-article","created":{"date-parts":[[2007,10,5]],"date-time":"2007-10-05T09:37:57Z","timestamp":1191577077000},"page":"104-110","source":"Crossref","is-referenced-by-count":47,"title":["Sensitization to blackgram in patients with bronchial asthma and rhinitis: clinical evaluation and characterization of allergens"],"prefix":"10.1111","volume":"61","author":[{"given":"D.","family":"Kumari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Sridhara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Arora","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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