{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T15:52:16Z","timestamp":1758815536154},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,1,23]],"date-time":"2006-01-23T00:00:00Z","timestamp":1137974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Immunoglobulin (Ig)E\u2010sensitized persons with positive skin prick test, but no allergy symptoms, are classified as being asymptomatic skin sensitized (AS). The allergic type 1 disease is dependant on IgE binding to the high affinity IgE\u2010receptor (Fc<jats:italic>\u025b<\/jats:italic>RI) expressed on basophils and mast cells. However, a relationship between the AS status and Fc<jats:italic>\u025b<\/jats:italic>RI has not been investigated. We aimed to characterize basophils from AS by looking at histamine release (HR) (sensitivity and reactivity) and the Fc<jats:italic>\u025b<\/jats:italic>RI molecule, and compare it with nonatopic (NA) or allergic (A) persons.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Blood was obtained from NA (<jats:italic>n<\/jats:italic>\u2003=\u200314), grass and\/or birch A persons (<jats:italic>n<\/jats:italic>\u2003=\u200317) and mono\u2010sensitized grass or birch pollen AS (<jats:italic>n<\/jats:italic>\u2003=\u200312). The basophil sensitivity and reactivity were examined by anti\u2010IgE triggered HR. Surface expression of Fc<jats:italic>\u025b<\/jats:italic>RI and IgE were measured by flow cytometry, Fc<jats:italic>\u025b<\/jats:italic>RI<jats:italic>\u03b1<\/jats:italic> protein was identified using a radioimmunoassay and Western blot. mRNA coding for the classic Fc<jats:italic>\u025b<\/jats:italic>RI<jats:italic>\u03b2<\/jats:italic>\u2010chain and the truncated form (Fc<jats:italic>\u025b<\/jats:italic>RI<jats:italic>\u03b2<\/jats:italic><jats:sub>T<\/jats:sub>) were determined by real\u2010time PCR.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The AS group was less reactive than NA or A persons when triggered by anti\u2010IgE and had a significant higher number of nonresponders. However, there was no difference in sensitivity among the three groups and furthermore; the groups did not vary in Fc<jats:italic>\u025b<\/jats:italic>RI\u2010 and IgE\u2010surface expression, Fc<jats:italic>\u025b<\/jats:italic>RI<jats:italic>\u03b1<\/jats:italic>\u2010protein level or <jats:italic>\u03b2<\/jats:italic>\/<jats:italic>\u03b2<\/jats:italic><jats:sub>T<\/jats:sub> ratio.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Basophils from AS persons are less reactive and include more nonresponders than basophils from NA and A persons, but do not differ regarding the Fc<jats:italic>\u025b<\/jats:italic>RI molecule.<\/jats:p>","DOI":"10.1111\/j.1398-9995.2006.00991.x","type":"journal-article","created":{"date-parts":[[2006,1,23]],"date-time":"2006-01-23T18:07:38Z","timestamp":1138039658000},"page":"303-310","source":"Crossref","is-referenced-by-count":2,"title":["Are basophil histamine release and high affinity IgE receptor expression involved in asymptomatic skin sensitization?"],"prefix":"10.1111","volume":"61","author":[{"given":"B. M.","family":"Jensen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Assing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Hummelshoj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Glue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. S.","family":"Skov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. K.","family":"Poulsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,23]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.17.1.931"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.37"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00130832-200402000-00003"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00062752-200001000-00007"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.120"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(88)90199-6"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2003.03385.x"},{"key":"e_1_2_7_9_2","doi-asserted-by":"crossref","first-page":"1317","DOI":"10.4049\/jimmunol.118.4.1317","article-title":"Measurement of IgE on human basophils: relation to serum IgE and anti\u2010IgE\u2010induced histamine release","volume":"118","author":"Conroy MC","year":"1977","journal-title":"J Immunol"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109103"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.70.2.207"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.6.3427"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.3.1290"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2003.01653.x"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(01)00285-0"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80203-4"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81300-8"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00115-8"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1159\/000085464"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20041384"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1993.tb04756.x"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1985.tb02679.x"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1985.tb00219.x"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.114653"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.1998.00337.x"},{"key":"e_1_2_7_27_2","first-page":"441","article-title":"Comparison of basophil histamine release induced by the cross\u2010linking of IgE receptors","volume":"39","author":"Tanizaki Y","year":"1985","journal-title":"Acta Med Okayama"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(94)90345-X"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70399-4"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(90)90046-7"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2005.00807.x"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70367-2"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.10.5913"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni817"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(96)70196-3"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2006.00991.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2006.00991.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T18:50:32Z","timestamp":1696963832000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2006.00991.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,1,23]]},"references-count":34,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["10.1111\/j.1398-9995.2006.00991.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2006.00991.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,1,23]]}}}