{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:02:42Z","timestamp":1791288162298,"version":"4.3.0"},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T00:00:00Z","timestamp":1153353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Skin eosinophilia is a common feature of allergic skin diseases, but it is unclear how epidermal and dermal eosinophil infiltration is controlled. To investigate regulation of localization of eosinophils in skin, we examined the regulatory mechanisms of expression of eosinophil\u2010specific chemoattractants in dermal fibroblasts and epidermal keratinocytes.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> We analyzed production of eotaxin, eotaxin\u20102, eotaxin\u20103 and galectin\u20109 by dermal fibroblasts and epidermal keratinocytes in response to several stimuli <jats:italic>in vitro<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Dermal fibroblasts produced eotaxin and eotaxin\u20103 in response to stimulation by interleukin (IL)\u20104 and\/or tumor necrosis factor\u2010<jats:italic>\u03b1<\/jats:italic>. Similarly, IL\u20104 stimulated epidermal keratinocytes to secrete eotaxin\u20103. However, we did not detect eotaxin mRNA expression or protein secretion by keratinocytes stimulated <jats:italic>in vitro<\/jats:italic>. Interferon (IFN)\u2010<jats:italic>\u03b3<\/jats:italic> induced galectin\u20109 expression on dermal fibroblasts. Conversely, expression of galectin\u20109 on epidermal keratinocytes was dose\u2010dependently inhibited by IFN\u2010<jats:italic>\u03b3<\/jats:italic>. The immunohistochemical assays revealed that dermal fibroblasts (but not epidermal keratinocytes) in the lesional skin of psoriasis vulgaris (a Th1\u2010polarized disease) express significant levels of galectin\u20109.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Eotaxin\u20103 contributes to dermal and epidermal eosinophil infiltration in Th2\u2010polarized skin inflammation in which IL\u20104 is produced. In contrast, IFN\u2010<jats:italic>\u03b3<\/jats:italic>\u2010dominated inflammation appears to mediate eosinophil extravasation into the dermis and eosinophil adhesion to dermal fibroblasts via galectin\u20109 in association with decreased chemoattractant activity of epidermal galectin\u20109. The present results reveal a novel mechanism of dermal eosinophilia in IFN\u2010<jats:italic>\u03b3<\/jats:italic>\u2010mediated skin inflammation, and reflect concerted chemoattractant production involving dermal and\/or epidermal eosinophilia during changes in the local cytokine profile.<\/jats:p>","DOI":"10.1111\/j.1398-9995.2006.01130.x","type":"journal-article","created":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T07:41:26Z","timestamp":1153381286000},"page":"1385-1391","source":"Crossref","is-referenced-by-count":39,"title":["Regulatory mechanisms of galectin\u20109 and eotaxin\u20103 synthesis in epidermal keratinocytes: possible involvement of galectin\u20109 in dermal eosinophilia of Th1\u2010polarized skin inflammation"],"prefix":"10.1111","volume":"61","author":[{"given":"K.","family":"Igawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Satoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Hirashima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Yokozeki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,20]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.160.1.60"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.7.4507"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2001.01299.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.6.3216"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.7169"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119341"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(00)90187-8"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)61399-3"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2003.10.001"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0803379"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.904"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.3780040406"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.10.5912"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/12.3.191"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.27.16976"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.72.3.486"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(03)00178-9"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2002.01755.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI9199"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2005.06508.x"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2003.02273.x"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12371752"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1990.tb08264.x"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1998.02027.x"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12323524"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12345482"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.3.1548"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1159\/000058002"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2006.01130.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2006.01130.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T16:52:45Z","timestamp":1696956765000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2006.01130.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,7,20]]},"references-count":28,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2006,12]]}},"alternative-id":["10.1111\/j.1398-9995.2006.01130.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2006.01130.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,7,20]]}}}