{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:32:27Z","timestamp":1791318747226,"version":"4.3.1"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,5,14]],"date-time":"2007-05-14T00:00:00Z","timestamp":1179100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Cyclooxygenase\u20101 (COX\u20101) is a key enzyme involved in generation of prostanoids, important mediators and modulators of asthmatic inflammation. In a subpopulation of aspirin\u2010sensitive asthmatics (ASA) inhibition of COX\u20101 by nonsteroidal anti\u2010inflammatory drugs results in activation of inflammatory cells and development of symptoms. Alternatively spliced variants of COX\u20101 lacking 111\u2003bp from exon 9 were described previously but have never been identified in human leucocytes peripheral blood leucocytes (PBL) or upper airway epithelial cells. We aimed to assess the expression of spliced variants of COX\u20101 mRNA in PBLs from patients with asthma and in healthy subjects (HS) referring the expression to patients characteristics (including ASA\u2010sensitivity) and to aspirin\u2010triggered 15\u2010hydroxyeicosatetraenoic acid (15\u2010HETE) generation.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> The study included 30 patients with ASA, 30 patients with aspirin\u2010tolerant asthma (ATA) and 30 HS serving as controls. Nasal polyps for epithelial cell cultures were obtained from 10 patients with chronic rhinosinusitis. Expression of full length and spliced variants of COX\u20101 enzyme was detected by RT\u2010PCR and presented as the ratio of full\u2010length COX\u20101 to alternatively spliced COX\u20101 mRNA [COX\u20101 alternative splicing index (COX\u20101 AS index)]. Release of eicosanoids (PGE<jats:sub>2<\/jats:sub> and 15\u2010HETE) by PBLs was measured with enzyme immunoassay.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> In both PBLs and airway epithelial cells the expression of full\u2010length product prevailed over spliced variants of COX\u20101 enzyme. Cyclooxygenase\u20101 AS index was significantly lower in asthmatics as compared to HS (1.96\u2003\u00b1\u20030.71 <jats:italic>vs<\/jats:italic> 2.41\u2003\u00b1\u20030.99, <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05) indicating the relatively higher expression of the alternative transcript in asthmatic patients. Cyclooxygenase\u20101 AS index was not different between ASA and ATA groups (mean 1.90\u2003\u00b1\u20030.66 <jats:italic>vs<\/jats:italic> 2.02\u2003\u00b1\u20030.76, respectively, <jats:italic>P<\/jats:italic>\u2003=\u20030.39). There was no significant association between COX\u20101 AS index and mean daily dose of inhaled glucocorticosteroids or pulmonary function tests (FEV<jats:sub>1<\/jats:sub>, FVC) but in ASA group a weak correlation with daily dose of oral glucocorticosteroids was found (<jats:italic>r<\/jats:italic>\u2003=\u20030.39; <jats:italic>P<\/jats:italic>\u2003=\u20030.03). In ASA patients there was a significant positive correlation between the COX\u20101 AS index and the percentage of aspirin\u2010triggered increase in 15\u2010HETE generation (<jats:italic>r<\/jats:italic>\u2003=\u20030.51; <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.03).<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Alternatively spliced variants of COX\u20101 mRNA are differently expressed in patients with bronchial asthma and may be associated with aspirin\u2010triggered 15\u2010HETE generation.<\/jats:p>","DOI":"10.1111\/j.1398-9995.2007.01366.x","type":"journal-article","created":{"date-parts":[[2007,5,14]],"date-time":"2007-05-14T12:00:54Z","timestamp":1179144054000},"page":"628-634","source":"Crossref","is-referenced-by-count":21,"title":["Alternative splicing of cyclooxygenase\u20101 gene: altered expression in leucocytes from patients with bronchial asthma and association with aspirin\u2010induced 15\u2010HETE release"],"prefix":"10.1111","volume":"62","author":[{"given":"M. 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