{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T18:10:04Z","timestamp":1789236604720,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,1,4]],"date-time":"2008-01-04T00:00:00Z","timestamp":1199404800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold>  <jats:italic>Anisakis simplex<\/jats:italic> allergens may cause severe allergic reactions in infected patients. Human anisakiasis can be specifically diagnosed by detection of immunoglobulin E (IgE) antibodies against <jats:italic>O<\/jats:italic>\u2010deglycosylated nAni s 7 allergen captured by monoclonal antibody (mAb) UA3 (UA3\u2010ELISA), although the nature of this important allergen is unknown. The aim of this study was to clone and characterize the Ani s 7 major allergen, and to obtain a recombinant fragment suitable for serodiagnosis.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> An <jats:italic>Anisakis<\/jats:italic> cDNA library was screened with mAb UA3 and a cDNA clone (rAni s 7) encoding a 1096\u2010amino acid fragment of Ani s 7 (GenBank: <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"DDBJ\/EMBL\/GenBank\" xlink:href=\"EF158010\">EF158010<\/jats:ext-link>) was identified. Bioinformatic tools and immunological and biochemical techniques were used to characterize the allergen obtained.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The rAni s 7 fragment comprised 19 repeats of a novel C<jats:italic>X<\/jats:italic><jats:sub>17\u221225<\/jats:sub>C<jats:italic>X<\/jats:italic><jats:sub>9\u221222<\/jats:sub>C<jats:italic>X<\/jats:italic><jats:sub>8<\/jats:sub>C<jats:italic>X<\/jats:italic><jats:sub>6<\/jats:sub> tandem repeat motif not seen in any previously reported protein sequence. An internal <jats:sup>435<\/jats:sup>Met\u2013<jats:sup>713<\/jats:sup>Arg fragment of the rAni s 7 (t\u2010Ani s 7) was expressed in <jats:italic>Escherichia coli<\/jats:italic> and evaluated for serodiagnostic utility. Indirect enzyme\u2010linked immunosorbent assay (ELISA) with t\u2010Ani s 7 identified as positive the same 60 sera as UA3\u2010ELISA. The sequence MCQCVQKYGTEFCKKRLA from rAni s 7 was identified as the epitope recognized by mAb UA3, and is the target for over 60% of human IgE antibodies that react with <jats:italic>O<\/jats:italic>\u2010deglycosylated nAni s 7.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> In addition to their clear value for serodiagnosis of human anisakiasis, the nature of the novel sequences and epitopes identified in the Ani s 7 allergen are of interest for a better understanding of the mechanisms operating in <jats:italic>Anisakis<\/jats:italic>\u2010induced allergy.<\/jats:p>","DOI":"10.1111\/j.1398-9995.2007.01564.x","type":"journal-article","created":{"date-parts":[[2010,8,17]],"date-time":"2010-08-17T12:24:52Z","timestamp":1282047892000},"page":"219-225","source":"Crossref","is-referenced-by-count":54,"title":["Novel sequences and epitopes of diagnostic value derived from the <i>Anisakis simplex<\/i> Ani s 7 major allergen*"],"prefix":"10.1111","volume":"63","author":[{"given":"E.","family":"Rodr\u00edguez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. M.","family":"Anad\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Garc\u00eda\u2010Bodas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Romar\u00eds","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Iglesias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"G\u00e1rate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. M.","family":"Ubeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,1,4]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(01)02152-3"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.07.013"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/161.5.995"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00012529"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1999.00574.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2000.00471.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2000.00758.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.106732"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2004.01.007"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)61914-X"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0396-4"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2007.01.007"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2001.00798.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1111\/j.1365-4632.2006.03091.x","article-title":"Cross\u2010reactivity of Anisakis simplex: possible role of Ani s 2 and Ani s 3","volume":"46","author":"Guarnieri F","year":"2007","journal-title":"Int J Dermatol"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050335"},{"key":"e_1_2_6_17_2","first-page":"105","volume-title":"A practical guide to monoclonal antibodies","author":"Liddel JE","year":"1991"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-005-0236-7"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.15.7583"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_6_21_2","volume-title":"Evaluation of allergenicity of genetically modified foods: report of a Joint FAO\/WHO Expert Consultation on allergenicity of foods derived from Biotechnology","author":"FAO and WHO","year":"2001"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.46.30801"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1033.2002.02990.x"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2003.11.002"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.2001.4392"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1991.tb01565.x"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2004.03.032"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2007.01564.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2007.01564.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T07:18:47Z","timestamp":1697440727000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2007.01564.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1,4]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2008,2]]}},"alternative-id":["10.1111\/j.1398-9995.2007.01564.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2007.01564.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,1,4]]}}}