{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T19:57:50Z","timestamp":1791662270189,"version":"4.3.4"},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2008,8,6]],"date-time":"2008-08-06T00:00:00Z","timestamp":1217980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> Sialic acid\u2010binding immunoglobulin\u2010like lectins (Siglecs) are a family of receptors that bind sialic acid and mostly contain immunoreceptor tyrosine\u2010based inhibitory motifs, suggesting that these molecules possess inhibitory functions. We have recently identified Siglec\u20108 as an eosinophil\u2010prominent Siglec, and cross\u2010linking of Siglec\u20108 on human eosinophils induces apoptosis. In this article, we address the <jats:italic>in vivo<\/jats:italic> consequences of Siglec engagement. We and others have identified mouse Siglec\u2010F as the closest functional paralog of human Siglec\u20108, based on shared ligand\u2010binding and expression pattern. We therefore hypothesized that Siglec\u2010F engagement would affect levels and viability of eosinophils <jats:italic>in vivo<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Wild type and hypereosinophilic mice were administered Siglec\u2010F antibody and levels of eosinophils in peripheral blood and tissue were measured. Eosinophil apoptosis (<jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>) was determined by binding of Annexin\u2010V.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Studies in IL\u20105 transgenic mice, displaying hypereosinophilia, show that administration of a single dose of Siglec\u2010F antibody results in rapid reductions in quantum of eosinophils in the blood. This decrease was accompanied by reductions in tissue eosinophils. Quantum of eosinophils in blood was decreased using two separate antibodies, as well as in other mouse models (wild type mice and in a mouse model of chronic eosinophilic leukemia). Mechanistic studies demonstrated that Siglec\u2010F antibody administration induced apoptosis of eosinophils <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> These data demonstrate that activation of innate immune receptors, like Siglec\u2010F, can significantly reduce mouse eosinophil viability. As such, targeting Siglec\u20108\/F may be a therapeutic approach for eosinophilic disorders.<\/jats:p>","DOI":"10.1111\/j.1398-9995.2008.01709.x","type":"journal-article","created":{"date-parts":[[2008,8,6]],"date-time":"2008-08-06T08:32:10Z","timestamp":1218011530000},"page":"1156-1163","source":"Crossref","is-referenced-by-count":124,"title":["Siglec\u2010F antibody administration to mice selectively reduces blood and tissue eosinophils"],"prefix":"10.1111","volume":"63","author":[{"given":"N.","family":"Zimmermann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. L.","family":"McBride","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. A.","family":"Hudson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. D.","family":"Cromie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. R.","family":"Crocker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. E.","family":"Rothenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. S.","family":"Bochner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,8,6]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199805283382206"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.11.6273"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(199811)252:3<418::AID-AR10>3.0.CO;2-1"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.2174\/1568010054526395"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa025217"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0932698100"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwj008"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2056"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.107127"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.2.861"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-10-3058"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.08.202"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2007-0154OC"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.10.004"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324723"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0888-7543(03)00171-X"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M412378200"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwi097"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1027\/0838-1925.19.2.54"},{"key":"e_1_2_6_21_2","first-page":"A690","volume-title":"Anti\u2010Siglec\u2010F antibody treatment during allergen\u2010induced airway inflammation reduces eosinophil numbers but has no effect on airway hyperreactivity in vivo","author":"Kearley J","year":"2007"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-08-039255"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.172.5.1425"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00383-07"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.116121"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-08-3153"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-6108(03)00108-9"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(84)90103-2"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(85)90151-8"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2006.12.542"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.01.023"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.53.4.347"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/7.6.631"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-10-4112"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2008.01709.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2008.01709.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T04:25:39Z","timestamp":1696911939000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2008.01709.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,8,6]]},"references-count":33,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2008,9]]}},"alternative-id":["10.1111\/j.1398-9995.2008.01709.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2008.01709.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,8,6]]}}}