{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T06:01:45Z","timestamp":1793944905587,"version":"4.8.0"},"reference-count":23,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2009,2,27]],"date-time":"2009-02-27T00:00:00Z","timestamp":1235692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:p>\n                    <jats:bold>Background:\u2002<\/jats:bold>\n                    The most prevalent drug hypersensitivity reactions are T\u2010cell mediated. The only established\n                    <jats:italic>in vitro<\/jats:italic>\n                    test for detecting T\u2010cell sensitization to drugs is the lymphocyte transformation test, which is of limited practicability. To find an alternative\n                    <jats:italic>in vitro<\/jats:italic>\n                    method to detect drug\u2010sensitized T cells, we screened the\n                    <jats:italic>in vitro<\/jats:italic>\n                    secretion of 17 cytokines\/chemokines by peripheral blood mononuclear cells (PBMC) of patients with well\u2010documented drug allergies, in order to identify the most promising cytokines\/chemokines for detection of T\u2010cell sensitization to drugs.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:\u2002<\/jats:bold>\n                    Peripheral blood mononuclear cell of 10 patients, five allergic to \u03b2\u2010lactams and five to sulfanilamides, and of five healthy controls were incubated for 3\u2003days with the drug antigen. Cytokine concentrations were measured in the supernatants using commercially available 17\u2010plex bead\u2010based immunoassay kits.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:\u2002<\/jats:bold>\n                    Among the 17 cytokines\/chemokines analysed, interleukin\u20102 (IL\u20102), IL\u20105, IL\u201013 and interferon\u2010\u03b3 (IFN\u2010\u03b3) secretion in response to the drugs were significantly increased in patients when compared with healthy controls. No difference in cytokine secretion patterns between sulfonamide\u2010 and \u03b2\u2010lactam\u2010reactive PBMC could be observed. The secretion of other cytokines\/chemokines showed a high variability among patients.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:\u2002<\/jats:bold>\n                    The measurement of IL\u20102, IL\u20105, IL\u201013 or IFN\u2010\u03b3 or a combination thereof might be a useful\n                    <jats:italic>in vitro<\/jats:italic>\n                    tool for detection of T\u2010cell sensitization to drugs. Secretion of these cytokines seems independent of the type of drug antigen and the phenotype of the drug reaction. A study including a higher number of patients and controls will be needed to determine the exact sensitivity and specificity of this test.\n                  <\/jats:p>","DOI":"10.1111\/j.1398-9995.2009.01985.x","type":"journal-article","created":{"date-parts":[[2009,2,27]],"date-time":"2009-02-27T05:07:54Z","timestamp":1235711274000},"page":"1269-1278","source":"Crossref","is-referenced-by-count":86,"title":["Drug\u2010specific\n                    <i>in vitro<\/i>\n                    release of IL\u20102, IL\u20105, IL\u201013 and IFN\u2010\u03b3 in patients with delayed\u2010type drug hypersensitivity"],"prefix":"10.1111","volume":"64","author":[{"given":"P.","family":"Lochmatter","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Beeler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. T.","family":"Kawabata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. O.","family":"Gerber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. J.","family":"Pichler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,9,14]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-139-8-200310210-00012"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1159\/isbn.978-3-318-01454-9"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.iac.2004.03.012"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2003.00280.x"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2004.00678.x"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2003.00279.x"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2004.00547.x"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2007.01516.x"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2004.12.026"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.04021.x"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2000.00905.x"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2002.01382.x"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2004.05.006"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-4362.1999.00792.x"},{"key":"e_1_2_7_16_2","first-page":"59","article-title":"In vitro interferon\u2010gamma release test in the diagnosis of drug\u2010induced erythema nodosum","volume":"6","author":"Halevy S","year":"2004","journal-title":"Isr Med Assoc J"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0536.2001.450601.x"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.10.030"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1997.tb00690.x"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2007.08.016"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.1919.x"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1586\/1744666X.2.6.887"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2230.2000.00729.x"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.155.1.462"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2009.01985.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2009.01985.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T11:43:34Z","timestamp":1696938214000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2009.01985.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9]]},"references-count":23,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2009,9]]}},"alternative-id":["10.1111\/j.1398-9995.2009.01985.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2009.01985.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9]]}}}