{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:28:47Z","timestamp":1791156527061,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2010,11,8]],"date-time":"2010-11-08T00:00:00Z","timestamp":1289174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:sec><jats:label\/><jats:p> <jats:bold>To cite this article:<\/jats:bold> Schiavi E, Barletta B, Butteroni C, Corinti S, Boirivant M, Di Felice G. Oral therapeutic administration of a probiotic mixture suppresses established Th2 responses and systemic anaphylaxis in a murine model of food allergy. <jats:italic>Allergy<\/jats:italic> 2011; <jats:bold>66<\/jats:bold>: 499\u2013508.<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background:\u2002<\/jats:bold> No effective treatment is available for food allergy and its primary management still consists of avoiding relevant allergens. Probiotics are claimed to beneficially affect the immune system. We sought to investigate the therapeutic potential of VSL#3 probiotic mixture on specific immune responses and anaphylactic reaction induced in mice by the major food allergen shrimp tropomyosin (ST).<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> The cytokine production by spleen cell from ST\u2010sensitized mice upon allergen re\u2010stimulation in the presence of VSL#3 was analysed. Next, the effects of oral administration of VSL#3 on allergen\u2010induced anaphylaxis and Th2 response in the murine model of food allergy to ST was investigated by evaluating symptom score and histamine content in the faeces after allergen challenge, antibody response in serum and faeces, and cytokine and transcription factor expression in the jejunum.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The <jats:italic>in vitro<\/jats:italic> studies on mouse spleen cells indicates that the VSL#3 preparation has the capacity to shift a polarized Th2 response to a Th1\/T regulatory\u2010type profile. Oral therapeutic administration of VSL#3 to ST\u2010sensitized mice significantly reduces symptom score and histamine release in the faeces following allergen challenge, as well as specific IgE response. In the jejunum, IL\u20104, IL\u20105 and IL\u201013 tissue content was significantly reduced, whereas FOXP3 and IL\u201027 mRNA expression, IL\u201010, TGF\u2010\u03b2 and IFN\u2010\u03b3 tissue content were up\u2010regulated.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Oral therapeutic treatment with the probiotic mixture VSL#3 is effective in redirecting allergen\u2010specific Th2\u2010polarized immune responses towards Th1\u2010T regulatory responses and in the protection against anaphylactic reactions induced by the allergen in a murine model of food allergy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1398-9995.2010.02501.x","type":"journal-article","created":{"date-parts":[[2010,11,8]],"date-time":"2010-11-08T15:53:15Z","timestamp":1289231595000},"page":"499-508","source":"Crossref","is-referenced-by-count":114,"title":["Oral therapeutic administration of a probiotic mixture suppresses established Th2 responses and systemic anaphylaxis in a murine model of food allergy"],"prefix":"10.1111","volume":"66","author":[{"given":"E.","family":"Schiavi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Barletta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Butteroni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Corinti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Boirivant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Di Felice","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,11,8]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.02.030"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.11.008"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2006.00431.x"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2005.05.006"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1998.028s5045.x"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.08.028"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1159\/000024201"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1159\/000236802"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1159\/000048166"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.118130"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.111237"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.02.010"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0b013e3282ffb134"},{"key":"e_1_2_7_15_2","unstructured":"Joint FAO\/WHO Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food. 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