{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T14:23:25Z","timestamp":1772893405520,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,9,7]],"date-time":"2011-09-07T00:00:00Z","timestamp":1315353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:sec><jats:label\/><jats:p><jats:bold>To cite this article:<\/jats:bold> Keswani A, Chustz RT, Suh L, Carter R, Peters AT, Tan BK, Chandra R, Kim S\u2010H, Azam T, Dinarello CA, Kern RC, Schleimer RP, Kato A. Differential expression of interleukin\u201032 in chronic rhinosinusitis with and without nasal polyps. <jats:italic>Allergy<\/jats:italic> 2012; <jats:bold>67<\/jats:bold>: 25\u201332.<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background:\u2002<\/jats:bold> Chronic rhinosinusitis (CRS) is a heterogeneous disease characterized by local inflammation of the upper airways and sinuses and is frequently divided into polypoid CRS (CRSwNP) and nonpolypoid CRS (CRSsNP). However, the mechanism of inflammation in CRS has still not been fully elucidated. The aim of the study was to investigate the role of interleukin\u201032 (IL\u201032), a recently discovered proinflammatory cytokine, in CRS.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> We collected nasal epithelial cells and nasal tissue from patients with CRS and control subjects. We assayed mRNA for IL\u201032 by real\u2010time PCR and measured IL\u201032 protein using ELISA, Western blot, and immunohistochemistry.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The expression of mRNA for IL\u201032 was elevated in epithelial cells from uncinate tissue from patients with CRSsNP compared with patients with CRSwNP (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05), control subjects (<jats:italic>P<\/jats:italic>\u2003=\u20030.06), and epithelial cells from nasal polyp (NP) tissue (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). Production of IL\u201032 was induced by IFN\u2010\u03b3, TNF, and dsRNA in primary airway epithelial cells. In whole\u2010tissue extracts, the expression of IL\u201032 protein was significantly elevated in patients with CRSwNP compared with patients with CRSsNP and control subjects. Immunohistochemistry data showed that IL\u201032 was detected in mucosal epithelial cells and inflammatory cells in the lamina propria. Levels of IL\u201032 were correlated with the levels of CD3 and macrophage mannose receptor in NP tissue. Immunofluorescence data showed IL\u201032 co\u2010localization with CD3\u2010positive T cells and CD68\u2010positive macrophages in NPs.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Overproduction of IL\u201032 may be involved in the pathogenesis of CRS, although the role of IL\u201032 in the inflammation in CRSsNP and CRSwNP may be different.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1398-9995.2011.02706.x","type":"journal-article","created":{"date-parts":[[2011,9,7]],"date-time":"2011-09-07T13:21:16Z","timestamp":1315401676000},"page":"25-32","source":"Crossref","is-referenced-by-count":60,"title":["Differential expression of interleukin\u201032 in chronic rhinosinusitis with and without nasal polyps"],"prefix":"10.1111","volume":"67","author":[{"given":"A.","family":"Keswani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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