{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T05:46:14Z","timestamp":1769147174619,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,12,17]],"date-time":"2011-12-17T00:00:00Z","timestamp":1324080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2012,3]]},"abstract":"Abstract<\/jats:title>Background:<\/jats:title>Eosinophils and lymphocytes are pathogenically important in allergic inflammation and sensitive to F<\/jats:styled-content>as\u2010mediated apoptosis. F<\/jats:styled-content>as ligand (FasL<\/jats:roman><\/jats:styled-content>) activity therefore should play a role in regulating the allergic immune response. We aimed to characterize the role of FasL<\/jats:roman><\/jats:styled-content> expression in airway eosinophilia in A<\/jats:italic><\/jats:styled-content>spergillus fumigatus<\/jats:italic> (A<\/jats:italic><\/jats:styled-content>f<\/jats:italic>)\u2010induced sensitization and to determine whether FasL<\/jats:roman><\/jats:styled-content> neutralization alters the inflammatory response.<\/jats:p><\/jats:sec>Methods:<\/jats:title>Sensitized B<\/jats:styled-content>alb\/c mice were killed before (day 0) and 1, 7 and 10\u00a0days after a single intranasal challenge with A<\/jats:italic><\/jats:styled-content>f<\/jats:italic>. Animals received either neutralizing antibody to FasL<\/jats:roman><\/jats:styled-content> (clone MFL<\/jats:styled-content>4) or irrelevant hamster I<\/jats:styled-content>gG <\/jats:styled-content>via<\/jats:italic> intraperitoneal injection on days \u22121 and 5. FasL<\/jats:roman><\/jats:styled-content> expression, BAL<\/jats:styled-content> and tissue inflammatory cell and cytokine profile, and apoptosis were assessed.<\/jats:p><\/jats:sec>Results:<\/jats:title>Postchallenge FasL<\/jats:roman><\/jats:styled-content> gene expression in BAL<\/jats:styled-content> cells and TUNEL<\/jats:styled-content> positivity in the airways coincided with the height of inflammatory cell influx on day 1, while soluble FasL<\/jats:roman><\/jats:styled-content> protein was released on day 7, preceding resolution of the inflammatory changes. Although eosinophil numbers showed a negative correlation with soluble FasL<\/jats:roman><\/jats:styled-content> levels in the airways, MBP<\/jats:styled-content>+<\/jats:sup> eosinophils remained TUNEL<\/jats:styled-content> negative in the submucosal tissue, throughout the 10\u2010day period after A<\/jats:italic><\/jats:styled-content>f<\/jats:italic> challenge. Systemic FasL<\/jats:roman><\/jats:styled-content> neutralization significantly enhanced BAL<\/jats:styled-content> and tissue eosinophil counts. This effect was associated with increased activation of T<\/jats:styled-content> cells and release of IL<\/jats:styled-content>\u20105, IL<\/jats:styled-content>\u20109, and GM<\/jats:styled-content>\u2010CSF<\/jats:styled-content> in the BAL<\/jats:styled-content> fluid of mice, indicating an involvement of pro\u2010eosinophilic survival pathways.<\/jats:p><\/jats:sec>Conclusions:<\/jats:title>FasL<\/jats:roman><\/jats:styled-content> activity may play an active role in resolving eosinophilic inflammation through regulating T<\/jats:styled-content> cells and pro\u2010eosinophilic cytokine release during the allergic airway response.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1398-9995.2011.02763.x","type":"journal-article","created":{"date-parts":[[2011,12,17]],"date-time":"2011-12-17T07:21:58Z","timestamp":1324106518000},"page":"328-335","source":"Crossref","is-referenced-by-count":19,"title":["Systemic FasL<\/scp><\/scp> neutralization increases eosinophilic inflammation in a mouse model of asthma"],"prefix":"10.1111","volume":"67","author":[{"given":"S. K.","family":"Sharma","sequence":"first","affiliation":[{"name":"Pulmonary, Allergy and Critical Care Division University of Pennsylvania School of Medicine Philadelphia PA USA"},{"name":"Center for Translational Medicine Thomas Jefferson University Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. A.","family":"Almeida","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Thomas Jefferson University Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Kierstein","sequence":"additional","affiliation":[{"name":"Pulmonary, Allergy and Critical Care Division University of Pennsylvania School of Medicine Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Hortobagyi","sequence":"additional","affiliation":[{"name":"Pulmonary, Allergy and Critical Care Division University of Pennsylvania School of Medicine Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Pulmonary and Critical Care Thomas Jefferson University Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Larkin","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine Thomas Jefferson University Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Peterson","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine Thomas Jefferson University Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Yagita","sequence":"additional","affiliation":[{"name":"Juntendo University School of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. G.","family":"Zangrilli","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine Thomas Jefferson University Philadelphia PA USA"},{"name":"Division of Pulmonary and Critical Care Thomas Jefferson University Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Haczku","sequence":"additional","affiliation":[{"name":"Pulmonary, Allergy and Critical Care Division University of Pennsylvania School of Medicine Philadelphia PA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,12,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.179.3.873"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90326-L"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/375078a0"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90071-3"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81266-0"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81589-5"},{"key":"e_1_2_8_8_1","first-page":"1","article-title":"National surveillance for asthma\u2013United States, 1980\u20132004","volume":"56","author":"Moorman JE","year":"2007","journal-title":"MMWR Surveill Summ"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200102013440507"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.285.20.2594"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.162.6.ats9-00"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V86.4.1437.bloodjournal8641437"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/373441a0"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00062.2002"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.6.3557"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.8.3914"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2007.01587.x"},{"key":"e_1_2_8_18_1","first-page":"598","article-title":"Airway hyperresponsiveness, elevation of serum\u2010specific IgE and activation of T cells following allergen exposure in sensitized Brown\u2010Norway rats","volume":"85","author":"Haczku A","year":"1995","journal-title":"Immunology"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.19.4.3100"},{"issue":"1","key":"e_1_2_8_20_1","first-page":"L444","article-title":"Airway epithelial Fas ligand expression: potential role in modulating bronchial inflammation","volume":"274","author":"Gochuico BR","year":"1998","journal-title":"Am J Physiol"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2001.790115.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-008-0299-1"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051680"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2008-0454OC"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118364"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000053727"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302325"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-6-90"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.118288"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(95)80069-7"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(200001)182:1<41::AID-JCP5>3.0.CO;2-7"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200402-240OC"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2570"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.182.6.1777"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.19.5.3166"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.08.016"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2011.02763.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2011.02763.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T18:44:35Z","timestamp":1695494675000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2011.02763.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12,17]]},"references-count":35,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1111\/j.1398-9995.2011.02763.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2011.02763.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12,17]]}}}