{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:59:21Z","timestamp":1791291561038,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2011,12,22]],"date-time":"2011-12-22T00:00:00Z","timestamp":1324512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>Nasal polyposis, a chronic inflammatory disease affecting the upper airways, is a valuable and accessible model to investigate the mechanisms underlying chronic inflammation. The main objective of this study was to investigate a potential involvement of the unfolded protein response (<jats:styled-content style=\"fixed-case\">UPR<\/jats:styled-content>) in the context of oxidative stress and inflammation in nasal epithelial cells from nasal polyps (<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Epithelial cells from <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a020) and normal mucosa (Controls, <jats:italic>n<\/jats:italic>\u00a0=\u00a015) in primary culture were analyzed by global proteomic approach and cell biology techniques for the glucose\u2010regulated protein 78 (<jats:styled-content style=\"fixed-case\"><jats:roman>GRP78<\/jats:roman><\/jats:styled-content>), the spliced <jats:styled-content style=\"fixed-case\">X<\/jats:styled-content>\u2010box\u2010binding protein 1 (<jats:styled-content style=\"fixed-case\"><jats:roman>sXBP\u20101<\/jats:roman><\/jats:styled-content>), the glucose\u2010regulated protein 94 (<jats:styled-content style=\"fixed-case\"><jats:roman>GRP94<\/jats:roman><\/jats:styled-content>), and the calreticulin (immunoblot, mass spectrometry, immunocytochemistry).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Proteomics analysis of human nasal epithelial cells in culture revealed the activation of the unfolded protein response in <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>. Systematic cell biology and biochemical analysis of two markers (<jats:styled-content style=\"fixed-case\"><jats:roman>GRP78<\/jats:roman><\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> <jats:roman>sXBP\u20101<\/jats:roman><\/jats:styled-content>) in the presence and absence of oxidative stress in <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> showed a susceptibility of the unfolded protein response to oxidative stress compared to controls at least partially linked to an abnormal redox state of the protein disulfide\u2010isomerase 4. This unfolded protein response was correlated with mitochondrial depolarization and secretion of interleukin 8 (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108) and leukotriene B4 (<jats:styled-content style=\"fixed-case\">LTB<\/jats:styled-content>4) and was prevented by mitochondrial antioxidant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>We show the existence of <jats:styled-content style=\"fixed-case\">UPR<\/jats:styled-content> in nasal epithelial cells that is linked to oxidative stress leading to <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108 and <jats:styled-content style=\"fixed-case\">LTB<\/jats:styled-content>4 secretions. These mechanisms may participate in chronic inflammation in nasal polyposis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1398-9995.2011.02769.x","type":"journal-article","created":{"date-parts":[[2011,12,22]],"date-time":"2011-12-22T10:11:31Z","timestamp":1324548691000},"page":"403-412","source":"Crossref","is-referenced-by-count":35,"title":["Oxidative stress induces unfolding protein response and inflammation in nasal polyposis"],"prefix":"10.1111","volume":"67","author":[{"given":"L.","family":"Jeanson","sequence":"first","affiliation":[{"name":"INSERM U955 Cr\u00e9teil France"},{"name":"INSERM U845 Universit\u00e9 Paris Descartes Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Kelly","sequence":"additional","affiliation":[{"name":"INSERM U845 Universit\u00e9 Paris Descartes Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Coste","sequence":"additional","affiliation":[{"name":"INSERM U955 Cr\u00e9teil France"},{"name":"Universit\u00e9 Paris Est Cr\u00e9teil France"},{"name":"Service d'Oto\u2010Rhino\u2010Laryngologie et de Chirurgie Cervico\u2010Faciale H\u00f4pitaux Henri Mondor (AP\u2010HP) et Intercommunal Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. C.","family":"Guerrera","sequence":"additional","affiliation":[{"name":"INSERM U845 Universit\u00e9 Paris Descartes Paris France"},{"name":"Plateau Proteome Necker Universit\u00e9 Paris Descartes IFR94 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Fritsch","sequence":"additional","affiliation":[{"name":"INSERM U845 Universit\u00e9 Paris Descartes Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Nguyen\u2010Khoa","sequence":"additional","affiliation":[{"name":"INSERM U845 Universit\u00e9 Paris Descartes Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Baudouin\u2010Legros","sequence":"additional","affiliation":[{"name":"INSERM U845 Universit\u00e9 Paris Descartes Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.\u2010F.","family":"Papon","sequence":"additional","affiliation":[{"name":"INSERM U955 Cr\u00e9teil France"},{"name":"Universit\u00e9 Paris Est Cr\u00e9teil France"},{"name":"Service d'Oto\u2010Rhino\u2010Laryngologie et de Chirurgie Cervico\u2010Faciale H\u00f4pitaux Henri Mondor (AP\u2010HP) et Intercommunal Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Zadigue","sequence":"additional","affiliation":[{"name":"INSERM U955 Cr\u00e9teil France"},{"name":"Universit\u00e9 Paris Est Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Pruli\u00e8re\u2010Escabasse","sequence":"additional","affiliation":[{"name":"INSERM U955 Cr\u00e9teil France"},{"name":"Universit\u00e9 Paris Est Cr\u00e9teil France"},{"name":"Service d'Oto\u2010Rhino\u2010Laryngologie et de Chirurgie Cervico\u2010Faciale H\u00f4pitaux Henri Mondor (AP\u2010HP) et Intercommunal Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Amselem","sequence":"additional","affiliation":[{"name":"INSERM U933 Universit\u00e9 Pierre et Marie Curie\u2010Paris 6 UMR S933 Paris France"},{"name":"AP\u2010HP H\u00f4pital Armand Trousseau Service de G\u00e9n\u00e9tique et d'Embryologie m\u00e9dicales Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Escudier","sequence":"additional","affiliation":[{"name":"INSERM U933 Universit\u00e9 Pierre et Marie Curie\u2010Paris 6 UMR S933 Paris France"},{"name":"AP\u2010HP H\u00f4pital Armand Trousseau Service de G\u00e9n\u00e9tique et d'Embryologie m\u00e9dicales Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Edelman","sequence":"additional","affiliation":[{"name":"INSERM U845 Universit\u00e9 Paris Descartes Paris France"},{"name":"Plateau Proteome Necker Universit\u00e9 Paris Descartes IFR94 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,12,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCP.0b013e32832c975a"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2007.01323.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1875-9114.1997.tb03696.x"},{"key":"e_1_2_8_5_1","first-page":"116","article-title":"[Free radicals and antioxidants in otorhinolaryngology]","volume":"57","author":"Holecek V","year":"2008","journal-title":"Cesk Fysiol"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/147323000903700206"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2005.09.032"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2003.02172.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.2500\/ajra.2010.24.3381"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200905-0740OC"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anl.2009.10.005"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0030-6657(07)70495-X"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3003.2000.15b24.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200211000-00030"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00048.2004"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.MLG.0000199738.37455.55"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1052"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.10.1211"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchemneu.2003.08.007"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1593\/neo.91354"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.964702"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313724200"},{"key":"e_1_2_8_23_1","first-page":"33","article-title":"Integration of ER stress, oxidative stress and the inflammatory response in health and disease","volume":"3","author":"Zhang K","year":"2010","journal-title":"Int J Clin Exp Med"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2007.10.004"},{"key":"e_1_2_8_25_1","first-page":"S1","article-title":"N\u2010acetyl\u2010cysteine protects against DNA damage associated with lead toxicity in HepG2 cells","volume":"20","author":"Yedjou CG","year":"2010","journal-title":"Ethn Dis"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2008.07.001"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2011.55"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/10715760802582183"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M500019-MCP200"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80198-7"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.109.162032"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000242903.41158.a1"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11596"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.24.10703-10717.2004"},{"key":"e_1_2_8_35_1","doi-asserted-by":"crossref","first-page":"pii: a004184","DOI":"10.1101\/cshperspect.a004184","article-title":"The Ca2+ pumps of the endoplasmic reticulum and Golgi apparatus","volume":"3","author":"Vandecaetsbeek I","year":"2011","journal-title":"Cold Spring Harb Perspect Biol"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.2169\/internalmedicine.42.7"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20071259"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00382.2007"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.07.021"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2007-0221OC"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-8-229"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2011.02769.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2011.02769.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T18:44:57Z","timestamp":1695494697000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2011.02769.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12,22]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1111\/j.1398-9995.2011.02769.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2011.02769.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12,22]]}}}