{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:24:47Z","timestamp":1761863087448},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,2,17]],"date-time":"2012-02-17T00:00:00Z","timestamp":1329436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Treatment options for food allergy remain limited. Development of novel approaches for the prevention and\/or treatment of severe peanut allergy and other food allergies is urgently needed. The objective of this study was to test whether skin application of food allergen can be used as a prophylactic and\/or therapeutic intervention for food allergy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Balb\/<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> mice were given 5\u00a0weekly cutaneous application of complete peanut extract (<jats:styled-content style=\"fixed-case\">CPE<\/jats:styled-content>) or ovalbumin (<jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>) ranging from 10 to 1000\u00a0\u03bcg on the shaved back skin, followed by 5\u00a0weekly treatments with oral <jats:styled-content style=\"fixed-case\">CPE<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content> plus cholera toxin to induce allergic reactivity to the food. At various time points, the immunologic responses and allergic clinical manifestations to allergens were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Skin application of a 10\u20131000\u00a0\u03bcg dose of <jats:styled-content style=\"fixed-case\">CPE<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content> to structurally intact skin did not lead to allergic sensitization to peanut or <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>. Rather, cutaneous allergen application blocked, in a dose\u2010dependent fashion, the subsequent induction of the oral sensitization including inhibiting oral sensitization\u2010induced <jats:styled-content style=\"fixed-case\">CPE<\/jats:styled-content>\u2010specific <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>1, and <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>2a production, suppressing the peanut anaphylaxis, and modulating the oral sensitization\u2010promoted cytokine production. The cutaneous <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content> application also resulted in similar results as seen with <jats:styled-content style=\"fixed-case\">CPE<\/jats:styled-content> application.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Cutaneous application of intact skin with peanut or <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content> can block the development of orally induced corresponding food allergies, suggesting that allergic tolerance to peanuts and <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content> might be achieved via allergen cutaneous application.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1398-9995.2012.02798.x","type":"journal-article","created":{"date-parts":[[2012,2,17]],"date-time":"2012-02-17T10:22:52Z","timestamp":1329474172000},"page":"622-629","source":"Crossref","is-referenced-by-count":25,"title":["Prevention of oral food allergy sensitization via skin application of 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Immunology\/Allergy Department of Medicine David Geffen School of Medicine at UCLA Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hart and Louise Lyon Immunology Laboratory Section of Clinical Immunology\/Allergy Division of Pulmonary, Critical Care and Clinical Immunology\/Allergy Department of Medicine David Geffen School of Medicine at UCLA Los Angeles CA 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