{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:05:40Z","timestamp":1778717140348,"version":"3.51.4"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,3,22]],"date-time":"2012-03-22T00:00:00Z","timestamp":1332374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Intradermal skin testing of the clinically important antibiotics ciprofloxacin, clarithromycin, and rifampicin in the case of suspected allergies to antibiotics is poorly standardized. For clinical practice, standardized procedures and protocols are desired.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fifteen healthy volunteers were tested with different concentrations of the antibiotics as well as with appropriate controls. Test readings included wheal area measured by digital image analysis and blood flow increase measured by laser Doppler flowmetry (<jats:styled-content style=\"fixed-case\">LDF<\/jats:styled-content>). To reduce interpersonal variability, test results were normalized with the individual controls using a novel protocol.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Nonirritating concentrations of the three antibiotics (ciprofloxacin ~0.0067\u00a0mg\/ml, clarithromycin ~0.05\u00a0mg\/ml, rifampicin ~0.002\u00a0mg\/ml) could be defined for healthy volunteers. Laser <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>oppler flowmetry generates comparable results to wheal area measurement. Normalization of the test results is necessary and can be applied in a practical algorithm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Standardized skin testing to detect sensitization to broadly used nonbetalactam antibiotics was presented and should be applied in truly sensitized patients. This approach should help to minimize the inter\u2010 and intraindividual differences in reactivity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1398-9995.2012.02807.x","type":"journal-article","created":{"date-parts":[[2012,3,23]],"date-time":"2012-03-23T06:38:25Z","timestamp":1332484705000},"page":"647-652","source":"Crossref","is-referenced-by-count":48,"title":["Nonirritant intradermal skin test concentrations of ciprofloxacin, clarithromycin, and rifampicin"],"prefix":"10.1111","volume":"67","author":[{"given":"P.","family":"Bro\u017e","sequence":"first","affiliation":[{"name":"Department of Angiology University Hospital Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Th.","family":"Harr","sequence":"additional","affiliation":[{"name":"Allergology Unit Department of Dermatology University Hospital Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Hecking","sequence":"additional","affiliation":[{"name":"Department of Angiology University Hospital Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Grize","sequence":"additional","affiliation":[{"name":"Swiss Tropical and Public Health Institute University of Basel Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Scherer","sequence":"additional","affiliation":[{"name":"Allergology Unit Department of Dermatology University Hospital Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. A.","family":"Jaeger","sequence":"additional","affiliation":[{"name":"Department of Angiology University Hospital Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J.","family":"Bircher","sequence":"additional","affiliation":[{"name":"Allergology Unit Department of Dermatology University Hospital Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,3,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1046\/j.0105-4538.2001.00001.x-i8","article-title":"General considerations for skin test procedures in the diagnosis of drug hypersensitivity","volume":"57","author":"Brockow K","year":"2002","journal-title":"Allergy"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp043986"},{"key":"e_1_2_7_4_1","first-page":"12","article-title":"Scratch\u2010patch and patch testing in drug allergy \u2013 an assessment of specificity","volume":"13","author":"Hug K","year":"2003","journal-title":"J Investig Allergol Clin Immunol"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.10.028"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1385\/CRIAI:24:3:201"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-005-0049-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0536.2001.450502.x"},{"key":"e_1_2_7_9_1","first-page":"543","article-title":"Practice parameters for allergy diagnostic testing. Joint Task Force on Practice Parameters for the Diagnosis and Treatment of Asthma. The American Academy of Allergy, Asthma and Immunology and the American College of Allergy, Asthma and Immunology","volume":"75","author":"Bernstein IL","year":"1995","journal-title":"Ann Allergy Asthma Immunol"},{"key":"e_1_2_7_10_1","first-page":"209","article-title":"Patterns of blood flow in the microcirculation of the skin during the course of the tuberculin reaction in normal human subjects","volume":"58","author":"Swanson Beck J","year":"1986","journal-title":"Immunology"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703789"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(03)01783-4"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000091839"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2006.02596.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199906000-00022"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2010.03.010"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2012.02807.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2012.02807.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T02:07:22Z","timestamp":1698718042000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2012.02807.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,22]]},"references-count":15,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1111\/j.1398-9995.2012.02807.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2012.02807.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,22]]}}}