{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T01:27:19Z","timestamp":1771464439983,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,3,30]],"date-time":"2012-03-30T00:00:00Z","timestamp":1333065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Nasal polyposis (<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>) is a Th2\u2010skewed inflammatory disorder, but it is unclear what role regulatory <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells (<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010reg) play in disease pathology. We investigated the expression profiles of <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010reg and <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010helper\u2010cell\u2010associated genes and their response to glucocorticosteroid (<jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>) treatment in <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>hinese patients with <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Biopsies were obtained from 29 non\u2010treated <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> patients for comparison with inferior turbinates collected from healthy controls. In 13 patients, <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> samples were collected both before and after short\u2010term oral <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> treatment. Levels of <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> for <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell markers were determined by microarray and quantitative <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Cellular infiltrates were assessed by histo\u2010 and immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">FOXP<\/jats:styled-content>3<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010reg were increased in <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>\u2010na\u00efve <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>, and numbers were negatively correlated with eosinophil infiltration. Helios staining was not detected, suggesting that <jats:styled-content style=\"fixed-case\">FOXP<\/jats:styled-content>3<jats:sup>+<\/jats:sup> cells in <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> are not thymus\u2010derived <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010reg. Compared with controls, <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels corresponding to <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010reg genes were significantly increased in <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">FOXP<\/jats:styled-content>3, <jats:styled-content style=\"fixed-case\">TGFB<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>10, <jats:styled-content style=\"fixed-case\">SMAD<\/jats:styled-content>3, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>2<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>3), but transcription factors associated with Th2 (<jats:styled-content style=\"fixed-case\">GATA<\/jats:styled-content>3) or Th17 responses (<jats:styled-content style=\"fixed-case\">ROR<\/jats:styled-content>c) were significantly reduced. <jats:styled-content style=\"fixed-case\">FOXP<\/jats:styled-content>3 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels positively correlated with other <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010reg cell markers. Microarray analysis showed that most Th2\u2010related markers (e.g., Eotaxin\u20101, <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>13, and <jats:styled-content style=\"fixed-case\">CCL<\/jats:styled-content>18) were upregulated in <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>\u2010na\u00efve <jats:styled-content style=\"fixed-case\">NP <\/jats:styled-content><jats:italic>vs<\/jats:italic> controls. <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> therapy significantly suppressed eosinophilic inflammation in <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>, but did not significantly alter the expression levels of T\u2010reg\/Th2\u2010associated genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Upregulation of <jats:styled-content style=\"fixed-case\">FOXP<\/jats:styled-content>3<jats:sup>+<\/jats:sup>\u2010inducible <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010reg cells and downregulation of Th2 and Th17 markers in <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> indicate a regulatory response occurring at a site of persistent mucosal inflammation. However, immune regulation fails to control the underlying tissue pathology. Expression of T\u2010reg\/Th2 markers after <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> treatment was unaltered, suggesting that <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell\u2010driving <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> inflammatory mediators are <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> resistant.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1398-9995.2012.02811.x","type":"journal-article","created":{"date-parts":[[2012,4,3]],"date-time":"2012-04-03T08:51:10Z","timestamp":1333443070000},"page":"732-740","source":"Crossref","is-referenced-by-count":32,"title":["Expression profiles of regulatory and helper <scp>T<\/scp>\u2010cell\u2010associated genes in nasal polyposis"],"prefix":"10.1111","volume":"67","author":[{"given":"C. W.","family":"Li","sequence":"first","affiliation":[{"name":"Department of Otolaryngology, Yong Loo Lin School of Medicine National University of Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. K.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Qilu Hospital Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. Y.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The First Affiliated Medical Hospital of Sun Yat\u2010Sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z. B.","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The First Affiliated Medical Hospital of Sun Yat\u2010Sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. Y.","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Yong Loo Lin School of Medicine National University of Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. A.","family":"Curotto de Lafaille","sequence":"additional","affiliation":[{"name":"Singapore Immunology Network Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Qilu Hospital Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. Y.","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Yong Loo Lin School of Medicine National University of Singapore Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,3,30]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"1","article-title":"European position paper on rhinosinusitis and nasal polyps 2007","volume":"20","author":"Fokkens W","year":"2007","journal-title":"Rhinology"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2005.09.026"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.1412"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200940045"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1760"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.04.009"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.05.002"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0904028"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.02.018"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.05.017"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2007.02739.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2007.01420.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.09-3079"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.05.010"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.02.030"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(94)90379-4"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2008.106096"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.12.011"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2785"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903243"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0050329"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200903-0392OC"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.10.6286"},{"key":"e_1_2_9_26_1","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.4049\/jimmunol.163.3.1545","article-title":"Glucocorticosteroids inhibit mRNA expression for eotaxin, eotaxin\u20102, and monocyte\u2010chemotactic protein\u20104 in human airway inflammation with eosinophilia","volume":"163","author":"Jahnsen FL","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.08.021"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.07.008"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2012.02811.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2012.02811.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:39:42Z","timestamp":1698712782000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2012.02811.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,30]]},"references-count":27,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,6]]}},"alternative-id":["10.1111\/j.1398-9995.2012.02811.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2012.02811.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,30]]}}}