{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:36:50Z","timestamp":1789385810691,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,4,9]],"date-time":"2012-04-09T00:00:00Z","timestamp":1333929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Activated <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> lymphocytes and their interaction with resident tissue cells, particularly epithelium, play important roles in inflammatory processes in chronic rhinosinusitis (<jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>). <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201032 is a recently described cytokine, which is expressed in a variety of tissue cells and involved in the pathogenesis of several chronic inflammatory diseases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Human sinus epithelial cells were isolated from biopsies and stimulated with different cytokines, which play a role in the pathogenesis of <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201032 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression was analyzed using real\u2010time\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> IL<\/jats:styled-content>\u201032 protein was determined by <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>estern blot and flow cytometry as well as immunofluorescent staining in primary sinus epithelial cells and nasal biopsies from patients with <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content> and healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201032 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> was upregulated by <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 and <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 in primary sinus epithelial cells, whereas <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101 \u03b2, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20104, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201013, and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017 did not influence <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201032 expression. <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201032 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression was significantly higher in human primary sinonasal epithelial cells (<jats:styled-content style=\"fixed-case\">HSEC<\/jats:styled-content>s) cocultured with Th1 cells compared with <jats:styled-content style=\"fixed-case\">HSEC<\/jats:styled-content>s cocultured with <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>h0 or <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>h2 cells. <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201032 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression was significantly higher in biopsies from sinus epithelial tissue of <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content> patients with nasal polyps compared with healthy subjects (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.01). <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201032 was detected in biopsies from patients with <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>, whereas it was scarcely present in control tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The induction of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201032 by <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1, <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 and <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>h1 cells as well as its increased expression in sinus tissues from <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content> patients with nasal polyps demonstrated a potential role for <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201032 in the pathogenesis of <jats:styled-content style=\"fixed-case\">CRS<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1398-9995.2012.02820.x","type":"journal-article","created":{"date-parts":[[2012,4,9]],"date-time":"2012-04-09T06:55:30Z","timestamp":1333954530000},"page":"790-798","source":"Crossref","is-referenced-by-count":37,"title":["Regulation and expression of <scp>IL<\/scp>\u201032 in chronic rhinosinusitis"],"prefix":"10.1111","volume":"67","author":[{"given":"M. B.","family":"Soyka","sequence":"first","affiliation":[{"name":"Swiss Institute of Allergy and Asthma Research (SIAF) University of Zurich Davos Switzerland"},{"name":"Department of Otolaryngology Head and Neck Surgery University Hospital Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Treis","sequence":"additional","affiliation":[{"name":"Swiss Institute of Allergy and Asthma Research (SIAF) University of Zurich Davos Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Eiwegger","sequence":"additional","affiliation":[{"name":"Swiss Institute of Allergy and Asthma Research (SIAF) University of Zurich Davos Switzerland"},{"name":"Department of Pediatrics Medical University of Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Menz","sequence":"additional","affiliation":[{"name":"Hochgebirgsklinik Davos, Davos Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Zhang","sequence":"additional","affiliation":[{"name":"BioLegend San Diego CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Holzmann","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery University Hospital Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. A.","family":"Akdis","sequence":"additional","affiliation":[{"name":"Swiss Institute of Allergy and Asthma Research (SIAF) University of Zurich Davos Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Meyer","sequence":"additional","affiliation":[{"name":"Swiss Institute of Allergy and Asthma Research (SIAF) University of Zurich Davos Switzerland"},{"name":"Hochgebirgsklinik Davos, Davos Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,4,9]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"97","article-title":"EP3OS 2007: European position paper on rhinosinusitis and nasal polyps 2007. A summary for otorhinolaryngologists","volume":"45","author":"Fokkens W","year":"2007","journal-title":"Rhinology"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.55.suppl_2.S20"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.07.013"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2010.02381.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOO.0b013e328334f670"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2500\/ajr.2006.20.2899"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(95)70298-9"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(98)70333-1"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.01225.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.02.018"},{"key":"e_1_2_9_12_1","first-page":"54","article-title":"Chronic sinusitis and rhinitis: Clinical terminology Chronic Rhinosinusitis further supported","volume":"48","author":"Van Crombruggen K","year":"2010","journal-title":"Rhinology"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200310000-00027"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.10.072"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.11.050"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2007.12.017"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.01.016"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2009.04.017"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M900368200"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2007.03439.x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0511233103"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2008.03025.x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2004.12.003"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.04.013"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2899"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117408"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.04.019"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200838885"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0030277"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000168111.11802.9c"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2004.00621.x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2008.02917.x"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2012.02820.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2012.02820.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T04:38:58Z","timestamp":1698727138000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2012.02820.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4,9]]},"references-count":31,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,6]]}},"alternative-id":["10.1111\/j.1398-9995.2012.02820.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2012.02820.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4,9]]}}}