{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T11:39:16Z","timestamp":1793014756851,"version":"4.5.0"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,5,14]],"date-time":"2012-05-14T00:00:00Z","timestamp":1336953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2012,7]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Chronic rhinosinusitis (CRS<\/jats:styled-content>) is an inflammation of the nose and of the paranasal sinuses. The involvement of the respiratory epithelium in the mechanisms of CRS<\/jats:styled-content> is poorly understood.<\/jats:p><\/jats:sec>Aims<\/jats:title>Among proteins expressed by nasal epithelial cells in CRS<\/jats:styled-content>, IL<\/jats:styled-content>\u201019 may have key functions. We here aimed to determine the expression and regulation of IL<\/jats:styled-content>\u201019.<\/jats:p><\/jats:sec>Methods<\/jats:title>Nasal biopsies from normal subjects (n<\/jats:italic>\u00a0=\u00a012), subjects with CRS<\/jats:styled-content> but without nasal polyps (NP<\/jats:styled-content>) (CRS<\/jats:styled-content>sNP<\/jats:styled-content>, n<\/jats:italic>\u00a0=\u00a012) and with CRS<\/jats:styled-content> with NP<\/jats:styled-content> (CRS<\/jats:styled-content>wNP<\/jats:styled-content>, n<\/jats:italic>\u00a0=\u00a015) were collected. Human A<\/jats:styled-content>sthma G<\/jats:styled-content>ene A<\/jats:styled-content>rray and real\u2010time PCR<\/jats:styled-content> were used to evaluate gene expression, western blot analysis and immunohistochemistry for protein expression. Results for IL<\/jats:styled-content>\u201019 were confirmed by real\u2010time PCR<\/jats:styled-content>. The constitutive and stimulated (LPS<\/jats:styled-content>, TGF<\/jats:styled-content> \u03b2) expression of IL<\/jats:styled-content>\u201019 and cell proliferation were evaluated in a nasal epithelial cell line (RPMI<\/jats:styled-content> 2650).<\/jats:p><\/jats:sec>Results<\/jats:title>Human A<\/jats:styled-content>sthma G<\/jats:styled-content>ene A<\/jats:styled-content>rray showed an increased IL<\/jats:styled-content>\u201019 gene expression in NP<\/jats:styled-content> from patients with CRS<\/jats:styled-content> in comparison with normal subjects. Real\u2010time PCR<\/jats:styled-content> confirmed the IL<\/jats:styled-content>\u201019 mRNA<\/jats:styled-content> up\u2010regulation in patients with CRS<\/jats:styled-content>wNP<\/jats:styled-content> and showed an up\u2010regulation of IL<\/jats:styled-content>\u201019, at lower extent, in patients with chronic rhinosinusitis without nasal polyps (CRS<\/jats:styled-content>sNP<\/jats:styled-content>) in comparison with normal subjects. Western blot analysis confirmed that IL<\/jats:styled-content>\u201019 is increased also at protein level in patients with CRS<\/jats:styled-content>wNP<\/jats:styled-content> in comparison with normal subjects. In NP<\/jats:styled-content>, IL<\/jats:styled-content>\u201019 is highly expressed in the metaplastic nasal epithelium when compared to normal or hyperplastic epithelium. LPS<\/jats:styled-content> stimulation increased IL<\/jats:styled-content>\u201019 expression, and recombinant IL<\/jats:styled-content>\u201019 increased cell proliferation in nasal epithelial cells.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>IL<\/jats:styled-content>\u201019 is overexpressed in the epithelium in CRS<\/jats:styled-content>wNP<\/jats:styled-content> and increases epithelial cell proliferation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1398-9995.2012.02842.x","type":"journal-article","created":{"date-parts":[[2012,5,14]],"date-time":"2012-05-14T18:24:48Z","timestamp":1337019888000},"page":"878-886","source":"Crossref","is-referenced-by-count":35,"title":["Increased expression of IL<\/scp>\u201019 in the epithelium of patients with chronic rhinosinusitis and nasal polyps"],"prefix":"10.1111","volume":"67","author":[{"given":"E.","family":"Pace","sequence":"first","affiliation":[{"name":"Institute of Biomedicine and Molecular Immunology (IBIM) National Research Council (CNR) Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Scafidi","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine and Molecular Immunology (IBIM) National Research Council (CNR) Palermo Italy"},{"name":"Dipartimento di Biopatologia e Metodologie Biomediche Universit\u00e0 degli Studi di Palermo Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Di Bona","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine and Molecular Immunology (IBIM) National Research Council (CNR) Palermo Italy"},{"name":"Dipartimento di Biopatologia e Metodologie Biomediche Universit\u00e0 degli Studi di Palermo Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Siena","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine and Molecular Immunology (IBIM) National Research Council (CNR) Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Chiappara","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine and Molecular Immunology (IBIM) National Research Council (CNR) Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ferraro","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine and Molecular Immunology (IBIM) National Research Council (CNR) Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"La Grutta","sequence":"additional","affiliation":[{"name":"Environmental Health ARPA Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Gallina","sequence":"additional","affiliation":[{"name":"Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche\u2010 Sezione di Otorinolaringoiatria Universit\u00e0 degli Studi di Palermo Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Speciale","sequence":"additional","affiliation":[{"name":"Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche\u2010 Sezione di Otorinolaringoiatria Universit\u00e0 degli Studi di Palermo Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ballacchino","sequence":"additional","affiliation":[{"name":"Dipartimento di Biomedicina Sperimentale e Neuroscienze Cliniche\u2010 Sezione di Otorinolaringoiatria Universit\u00e0 degli Studi di Palermo Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Bachert","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Upper Airway Research Laboratory (URL) University Hospital Ghent Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Bousquet","sequence":"additional","affiliation":[{"name":"University Hospital 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