{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:23:54Z","timestamp":1791231834002,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,6,8]],"date-time":"2012-06-08T00:00:00Z","timestamp":1339113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2012,8]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Diagnostic tests in patients with H<\/jats:styled-content>ymenoptera venom allergy are frequently positive to venoms of both honey bee and wasp (V<\/jats:styled-content>espula<\/jats:italic>). Component\u2010resolved analysis with recombinant species\u2010specific major allergens (rSSMA<\/jats:styled-content>) may help to distinguish true double sensitization from crossreactivity.<\/jats:p><\/jats:sec>Methods<\/jats:title>Included were 121 patients with systemic allergic reactions to H<\/jats:styled-content>ymenoptera stings, 76 with double positivity of serum\u2010specific I<\/jats:styled-content>gE<\/jats:styled-content> (sI<\/jats:styled-content>gE<\/jats:styled-content>) to both venoms, 45 with single positivity to bee or wasp venom, and 32 controls without history of systemic reactions to H<\/jats:styled-content>ymenoptera stings and no sI<\/jats:styled-content>gE<\/jats:styled-content> to whole venoms. In venom\u2010allergic patients and controls, sI<\/jats:styled-content>gE<\/jats:styled-content> to rSSMA A<\/jats:styled-content>pi m 1 of bee venom and to V<\/jats:styled-content>es v 1 and V<\/jats:styled-content>es v 5 of wasp venom were tested by I<\/jats:styled-content>mmunoCAP<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>Only 47% of 76 patients with double positivity to whole venoms reacted also to rSSMA<\/jats:styled-content> of both species. Specificity of sI<\/jats:styled-content>gE<\/jats:styled-content> to the 3 rSSMA<\/jats:styled-content> was very high, with no sI<\/jats:styled-content>gE<\/jats:styled-content> to rSSMA<\/jats:styled-content> of the other species in single\u2010positive venom\u2010allergic patients and only one control with low sI<\/jats:styled-content>gE<\/jats:styled-content> to V<\/jats:styled-content>es v 1. All wasp\u2010allergic single\u2010positive patients had sI<\/jats:styled-content>gE<\/jats:styled-content> to V<\/jats:styled-content>es v 5 and\/or V<\/jats:styled-content>es v 1, and 78.3% of single\u2010positive bee venom\u2013allergic patients had sI<\/jats:styled-content>gE<\/jats:styled-content> to A<\/jats:styled-content>pi m 1.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Specificity of sI<\/jats:styled-content>gE<\/jats:styled-content> to rSSMA<\/jats:styled-content> of both species is excellent. Sensitivity of sI<\/jats:styled-content>gE<\/jats:styled-content> to rSSMA<\/jats:styled-content> was optimal for wasp venom. Sensitivity of bee venom A<\/jats:styled-content>pi m 1 could be increased by adding rSSMA<\/jats:styled-content> of other important bee venom allergens.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1398-9995.2012.02847.x","type":"journal-article","created":{"date-parts":[[2012,6,8]],"date-time":"2012-06-08T10:31:23Z","timestamp":1339151483000},"page":"1069-1073","source":"Crossref","is-referenced-by-count":107,"title":["IgE to recombinant allergens A<\/scp>pi m 1, V<\/scp>es v 1, and V<\/scp>es v 5 distinguish double sensitization from crossreaction in venom allergy"],"prefix":"10.1111","volume":"67","author":[{"given":"U.","family":"M\u00fcller","sequence":"first","affiliation":[{"name":"Division of Allergy, Department of Medicine Spital Ziegler Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Schmid\u2010Grendelmeier","sequence":"additional","affiliation":[{"name":"Allergy Unit, Department of Dermatology University Hospital of Z\u00fcrich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Hausmann","sequence":"additional","affiliation":[{"name":"Division of Allergy, Department of Medicine Spital Ziegler Bern Switzerland"},{"name":"Division of Allergy, Department of Rheumatology Clinical Immunology and Allergology, Inselspital, University of Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Helbling","sequence":"additional","affiliation":[{"name":"Division of Allergy, Department of Medicine Spital Ziegler Bern Switzerland"},{"name":"Division of Allergy, Department of Rheumatology Clinical Immunology and Allergology, Inselspital, University of Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,6,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2008.01794.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(81)90133-0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.01897.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02630.x"},{"key":"e_1_2_8_6_1","first-page":"237","volume-title":"Allergens and Allergen Immunotherapy","author":"King TP","year":"2008"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2009.01.017"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/02770906609106941"},{"key":"e_1_2_8_9_1","volume-title":"Insect Sting Allergy. Clinical Picture, Diagnosis and Treatment","author":"M\u00fcller UR","year":"1990"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.03.017"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020842"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803709"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.120013"},{"key":"e_1_2_8_14_1","first-page":"357","article-title":"Hymenoptera venom allergy: taking the sting out of difficult cases","volume":"17","author":"Ebo D","year":"2007","journal-title":"J Investig Allergol Clin Immunol"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2004.00624.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2004.01949.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2010.03535.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20419"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.06.042"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.03.012"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02781.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986611794653923"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2009.10.005"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.12.1331"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.08.005"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02667.x"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2012.02847.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2012.02847.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T17:54:38Z","timestamp":1700589278000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2012.02847.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,8]]},"references-count":25,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2012,8]]}},"alternative-id":["10.1111\/j.1398-9995.2012.02847.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2012.02847.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,8]]}}}