{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T15:39:47Z","timestamp":1769269187072,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,6,4]],"date-time":"2012-06-04T00:00:00Z","timestamp":1338768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Murine models suggest a critical functional role for the anti\u2010inflammatory cytokine <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 in local regulation of allergic airways inflammation. There is little corresponding information on human airway cells. This study aimed to investigate whether local <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 production regulates responses by respiratory mucosal leucocytes isolated from nasal polyps.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Nasal polyp tissue was harvested from 24 patients sensitised to aeroallergens with chronic rhinitis and polyposis undergoing routine polypectomy. Cells were isolated by matrix proteolysis. Cytokine production by polyp cells was determined by cytometric bead array (<jats:styled-content style=\"fixed-case\">CBA<\/jats:styled-content>) and intracellular cytokine analysis. Surface marker expression by polyp cells was determined by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Allergen stimulation significantly enhanced production of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010, but not <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20105 or <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 by nasal polyp cell suspensions. Under the same conditions, neutralisation of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 significantly increased allergen\u2010specific <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20105 and <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 production by nasal polyp cells. Cell depletion experiments showed that <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells themselves were primarily responsible for <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 production or for inducing its production by other cells. Intracellular cytokine staining confirmed production of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 in the absence of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 production by <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells in response to allergen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>T cells within the human respiratory mucosa produce <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010, which is capable of inhibiting pro\u2010inflammatory <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>h2 and <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>h1 cytokine production in an antigen\u2010specific fashion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1398-9995.2012.02852.x","type":"journal-article","created":{"date-parts":[[2012,6,4]],"date-time":"2012-06-04T19:59:56Z","timestamp":1338839996000},"page":"1007-1013","source":"Crossref","is-referenced-by-count":23,"title":["<scp>T<\/scp> cells producing the anti\u2010inflammatory cytokine <scp>IL<\/scp>\u201010 regulate allergen\u2010specific <scp>T<\/scp>h2 responses in human airways"],"prefix":"10.1111","volume":"67","author":[{"given":"A.","family":"Faith","sequence":"first","affiliation":[{"name":"MRC\u2010Asthma UK Centre in Allergic Mechanisms of Asthma King's College London School of Medicine UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Singh","sequence":"additional","affiliation":[{"name":"The Ear Nose and Throat Department Guy's and St Thomas' Hospitals King's College London London UK"},{"name":"University of Sydney Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Farooque","sequence":"additional","affiliation":[{"name":"MRC\u2010Asthma UK Centre in Allergic Mechanisms of Asthma King's College London School of Medicine UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Dimeloe","sequence":"additional","affiliation":[{"name":"MRC\u2010Asthma UK Centre in Allergic Mechanisms of Asthma King's College London School of Medicine UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. F.","family":"Richards","sequence":"additional","affiliation":[{"name":"MRC\u2010Asthma UK Centre in Allergic Mechanisms of Asthma King's College London School of Medicine UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Lu","sequence":"additional","affiliation":[{"name":"MRC\u2010Asthma UK Centre in Allergic Mechanisms of Asthma King's College London School of Medicine UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Roberts","sequence":"additional","affiliation":[{"name":"The Ear Nose and Throat Department Guy's and St Thomas' Hospitals King's College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Chevretton","sequence":"additional","affiliation":[{"name":"The Ear Nose and Throat Department Guy's and St Thomas' Hospitals King's College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. H.","family":"Lee","sequence":"additional","affiliation":[{"name":"MRC\u2010Asthma UK Centre in Allergic Mechanisms of Asthma King's College London School of Medicine UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. J.","family":"Corrigan","sequence":"additional","affiliation":[{"name":"MRC\u2010Asthma UK Centre in Allergic Mechanisms of Asthma King's College London School of Medicine UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. M.","family":"Hawrylowicz","sequence":"additional","affiliation":[{"name":"MRC\u2010Asthma UK Centre in Allergic Mechanisms of Asthma King's College London School of Medicine UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,6,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20032058"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1589"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15592-X"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.05.002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2007.02739.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.5.3252"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI2250"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830270513"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.01964.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02589.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"crossref","first-page":"6283","DOI":"10.4049\/jimmunol.163.11.6283","article-title":"Essential role for both CD80 and CD86 costimulation, but not CD40 interactions, in allergen\u2010induced Th2 cytokine production from asthmatic bronchial tissue: role for alphabeta, but not gammadelta, T cells","volume":"163","author":"Jaffar ZH","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.107398"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm745"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20060155"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000155739"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2007.01323.x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.08.002"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2009.02174.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20060772"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200636575"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(98)70333-1"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.08.018"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.02.017"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200310-1416OC"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)17704-6"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.7.4658"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.11.009"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1503"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-11-188847"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051166"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2012.02852.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2012.02852.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T17:54:15Z","timestamp":1700589255000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2012.02852.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6,4]]},"references-count":30,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2012,8]]}},"alternative-id":["10.1111\/j.1398-9995.2012.02852.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2012.02852.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6,4]]}}}