{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:06:32Z","timestamp":1791421592318,"version":"4.3.3"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,6,15]],"date-time":"2012-06-15T00:00:00Z","timestamp":1339718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2012,9]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>The regulation and function of I<\/jats:styled-content>gE<\/jats:styled-content> in healthy individuals and in antigen\u2010na\u00efve animals is not well understood. IL<\/jats:styled-content>\u201033 administration increases serum I<\/jats:styled-content>gE<\/jats:styled-content> in mice with unknown mechanism. We tested the hypothesis that IL<\/jats:styled-content>\u201033 provides an antigen\u2010independent stimulus for I<\/jats:styled-content>gE<\/jats:styled-content> production and mast cell degranulation.<\/jats:p><\/jats:sec>Methods<\/jats:title>IL<\/jats:styled-content>\u201033 was administered to na\u00efve wild\u2010type (WT<\/jats:styled-content>), nude and ST<\/jats:styled-content>2\u2212\/\u2212<\/jats:sup>, IL<\/jats:styled-content>\u20104\u2212\/\u2212<\/jats:sup>, IL<\/jats:styled-content>4R\u03b1\u2212\/\u2212<\/jats:sup> and T<\/jats:styled-content>\u2010or B<\/jats:styled-content>\u2010cell\u2010specific IL<\/jats:styled-content>\u20104R<\/jats:styled-content>\u03b1\u2212\/\u2212<\/jats:sup> mice. I<\/jats:styled-content>gE<\/jats:styled-content>and cytokines were quantified by ELISA<\/jats:styled-content>. T<\/jats:styled-content>\u2010 and B<\/jats:styled-content>\u2010lymphocyte numbers and CD<\/jats:styled-content>40L expression were determined by flow cytometry. Anaphylaxis was measured by temperature, mast cell degranulation and histamine release.<\/jats:p><\/jats:sec>Results<\/jats:title>IL<\/jats:styled-content>\u201033 enhanced I<\/jats:styled-content>gE<\/jats:styled-content> production in na\u00efve WT<\/jats:styled-content>, T\u2010IL<\/jats:styled-content>\u20104R<\/jats:styled-content>\u03b1\u2212\/\u2212<\/jats:sup> but not in ST<\/jats:styled-content>2\u2212\/\u2212<\/jats:sup>, IL<\/jats:styled-content>\u20104\u2212\/\u2212<\/jats:sup>, IL<\/jats:styled-content>\u20104R<\/jats:styled-content>\u03b1\u2212\/\u2212<\/jats:sup> or B<\/jats:styled-content>\u2010cell\u2010specific IL<\/jats:styled-content>\u20104R<\/jats:styled-content>\u03b1\u2212\/\u2212<\/jats:sup> mice, demonstrating IL<\/jats:styled-content>\u201033 specificity and IL<\/jats:styled-content>\u20104 dependency. Moreover, IL<\/jats:styled-content>\u20104 was required for IL<\/jats:styled-content>\u201033\u2010induced B<\/jats:styled-content>\u2010cell proliferation and T<\/jats:styled-content>\u2010cell CD<\/jats:styled-content>40L<\/jats:styled-content> expression, which promotes I<\/jats:styled-content>gE<\/jats:styled-content> production. IL<\/jats:styled-content>\u201033\u2010induced IL<\/jats:styled-content>\u20104 production was mainly from innate cells including mast cells and eosinophils. IL<\/jats:styled-content>\u201033 increased mast cell surface I<\/jats:styled-content>gE<\/jats:styled-content> and triggered degranulation and systemic anaphylaxis in allergen\u2010na\u00efve WT<\/jats:styled-content> but not in IL<\/jats:styled-content>\u20104R<\/jats:styled-content>\u03b1\u2212\/\u2212<\/jats:sup> mice.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>IL<\/jats:styled-content>\u201033 amplifies I<\/jats:styled-content>gE<\/jats:styled-content> synthesis and triggers anaphylaxis in na\u00efve mice via IL<\/jats:styled-content>\u20104, independent of allergen. IL<\/jats:styled-content>\u201033 may play an important role in nonatopic allergy and idiopathic anaphylaxis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1398-9995.2012.02859.x","type":"journal-article","created":{"date-parts":[[2012,6,17]],"date-time":"2012-06-17T21:39:34Z","timestamp":1339969174000},"page":"1118-1126","source":"Crossref","is-referenced-by-count":133,"title":["Interleukin\u201033 amplifies I<\/scp>gE<\/scp> synthesis and triggers mast cell degranulation via interleukin\u20104 in na\u00efve mice"],"prefix":"10.1111","volume":"67","author":[{"given":"M.","family":"Komai\u2010Koma","sequence":"first","affiliation":[{"name":"Institute of Infection, Immunity and Inflammation University of Glasgow Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Brombacher","sequence":"additional","affiliation":[{"name":"Institute of Infectious Disease and Molecular Medicine University of Cape Town and International Center for Genetic Engineering and Biotechnology Cape Town South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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