{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T17:08:11Z","timestamp":1793898491971,"version":"4.8.0"},"reference-count":12,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,7,30]],"date-time":"2012-07-30T00:00:00Z","timestamp":1343606400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Most cases of beer allergy reported so far have been associated with hypersensitivity to the non\u2010specific lipid transfer protein (<jats:styled-content style=\"fixed-case\">LTP<\/jats:styled-content>). In view of the marked differences in brewing processes we assessed IgE reactivity as well as tolerance to many different beers in an allergic patient.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A 45\u00a0year\u2010old man hypersensitive to grass pollen, cat dander and <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>lternaria tenuis<\/jats:italic> with a history of urticaria and dyspnoea after drinking beer and a weak skin reactivity to commercial corn extract was studied. The patient underwent <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content> with 36 different brands of beer and an open challenge with those scoring negative was performed. An immunoblot analysis was carried out using 2 <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content>\u2010positive beers, 2 <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content>\u2010negative beers, and barley, wheat, and maize extracts using both patient's serum and a maize <jats:styled-content style=\"fixed-case\">LTP<\/jats:styled-content>\u2010specific in\u2010house developed polyclonal antibody from rabbit. Further, the immune reactive <jats:styled-content style=\"fixed-case\">LTP<\/jats:styled-content> of one beer was separated by <jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> and the chromatogram was compared to that of purified maize <jats:styled-content style=\"fixed-case\">LTP<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Beer SPT scored positive in 30\/36 cases. The immunoblot analysis showed IgE reactivity at about 10\u00a0<jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a against the two <jats:styled-content style=\"fixed-case\">SPT<\/jats:styled-content>\u2010positive beers and against maize with both patient's serum and the polyclonal anti\u2010<jats:styled-content style=\"fixed-case\">LTP<\/jats:styled-content> rabbit serum, whereas the two SPT\u2010negative beers, and barley extract scored negative. The immunodetected protein co\u2010migrated with maize <jats:styled-content style=\"fixed-case\">LTP<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In beer\u2010allergic patients the diagnostic workup may point to the detection of some tolerated products that can be consumed risk\u2010free.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1398-9995.2012.02872.x","type":"journal-article","created":{"date-parts":[[2012,7,30]],"date-time":"2012-07-30T07:17:31Z","timestamp":1343632651000},"page":"1186-1189","source":"Crossref","is-referenced-by-count":20,"title":["Allergy to beer in <scp>LTP<\/scp>\u2010sensitized patients: beers are not all the same"],"prefix":"10.1111","volume":"67","author":[{"given":"O.","family":"Quercia","sequence":"first","affiliation":[{"name":"Allergy Unit Faenza Hospital Faenza"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Zoccatelli","sequence":"additional","affiliation":[{"name":"Department of Biotechnology University of Verona Verona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. F.","family":"Stefanini","sequence":"additional","affiliation":[{"name":"Allergy Unit Faenza Hospital Faenza"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Mistrello","sequence":"additional","affiliation":[{"name":"Lofarma SpA R &amp; D Milano"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Amato","sequence":"additional","affiliation":[{"name":"Lofarma SpA R &amp; D Milano"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Bolla","sequence":"additional","affiliation":[{"name":"Department of Biotechnology University of Verona Verona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Emiliani","sequence":"additional","affiliation":[{"name":"Allergy Unit Faenza Hospital Faenza"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Asero","sequence":"additional","affiliation":[{"name":"Ambulatorio di Allergologia Clinica San Carlo Paderno Dugnano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,7,30]]},"reference":[{"key":"e_1_2_2_2_1","first-page":"268","article-title":"Beer\u2010induced anaphylaxis due to barley sensitization: two case reports","volume":"9","author":"Bonadonna P","year":"1999","journal-title":"J Investig Allergol Clin Immunol"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1999.00491.x"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000024355"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)62325-3"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.118793"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.1999.00827.x"},{"key":"e_1_2_2_8_1","first-page":"121","article-title":"Food\u2010induced and occupational asthma due to barley flour","volume":"75","author":"Vidal C","year":"1995","journal-title":"Ann Allergy Asthma Immunol"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1994.tb01806.x"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1993.tb04756.x"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf000075m"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2002.t01-1-23541.x"},{"key":"e_1_2_2_13_1","volume-title":"Malts and Malting","author":"Briggs DE","year":"1998"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1398-9995.2012.02872.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1398-9995.2012.02872.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T13:30:37Z","timestamp":1695475837000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1398-9995.2012.02872.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7,30]]},"references-count":12,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2012,9]]}},"alternative-id":["10.1111\/j.1398-9995.2012.02872.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1398-9995.2012.02872.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7,30]]}}}