{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T16:03:39Z","timestamp":1774973019346,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,2,13]],"date-time":"2006-02-13T00:00:00Z","timestamp":1139788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Microbiology and Immunology"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> A connection of herpesvirus periapical infection with symptomatic and large\u2010size periapical lesions has been recognized in adult patients, but no data exist about a possible involvement of herpesviruses in severe periapical pathosis in children. Herpesviruses have the potential to elicit potent bone resorption\u2010inducing cytokines in mammalian cells.<\/jats:p><jats:p><jats:bold>Aim:\u2002<\/jats:bold> This study aimed to determine the occurrence of human cytomegalovirus and Epstein\u2010Barr virus DNA, and mRNA transcripts of receptor activator of nuclear kappa B ligand (RANKL), osteoprotegerin, core binding factor alpha\u20101, colony stimulating factor\u20101, transforming growth factor\u2010\u03b2, and monocyte chemoattractant protein\u20101 in periapical symptomatic pathosis of deciduous teeth.<\/jats:p><jats:p><jats:bold>Material and methods:\u2002<\/jats:bold> Twelve deciduous molar teeth from patients aged 2\u20138\u2003years were extracted due to severe periapical infection, and granulomatous tissue adherent to the root tip of the extracted teeth was collected using a surgical knife. Non\u2010diseased pulpal tissue, obtained from 12 teeth extracted for orthodontic reasons, served as negative control. Polymerase chain reaction assays were employed to identify herpesvirus DNA and cytokine gene expression, using established polymerase chain reaction primers and procedures.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Seven (58%) of the periapical lesions yielded human cytomegalovirus and eight (67%) Epstein\u2010Barr virus. Only one (8%) periapical lesion showed neither human cytomegalovirus nor Epstein\u2010Barr virus. In healthy pulpal tissue, one (8%) specimen demonstrated human cytomegalovirus and another (8%) specimen revealed Epstein\u2010Barr virus. Of the cytokines examined, RANKL expression showed significantly higher occurrence in periapical pathosis than in healthy pulpal tissue (<jats:italic>P\u2003&lt;<\/jats:italic>\u20030.040). No relationship was identified between the type of herpesvirus and cytokine expression in the periapical lesions studied.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> The present findings provide evidence of a putative role of human cytomegalovirus and Epstein\u2010Barr virus in the pathogenesis of symptomatic periapical pathosis in deciduous teeth. Increased RANKL expression in periapical lesions may be of pathogenetic significance.<\/jats:p>","DOI":"10.1111\/j.1399-302x.2006.00268.x","type":"journal-article","created":{"date-parts":[[2006,2,13]],"date-time":"2006-02-13T14:09:18Z","timestamp":1139839758000},"page":"107-111","source":"Crossref","is-referenced-by-count":33,"title":["Human cytomegalovirus, Epstein\u2010Barr virus and bone resorption\u2010inducing cytokines in periapical lesions of deciduous teeth"],"prefix":"10.1111","volume":"21","author":[{"given":"S.","family":"Yildirim","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Yapar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Kubar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Slots","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,2,13]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mn.7800190"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1067\/moe.2000.108958"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910570623"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5128"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001090100226"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2004.00773.x"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20036"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2004.02.007"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-001-2086-7"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.65.1.131-150.2001"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcbr.2000.0205"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.DON.0000134210.95473.A7"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302X.2003.00079.x"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302X.2003.00055.x"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2005.00232.x"},{"key":"e_1_2_5_17_2","doi-asserted-by":"crossref","unstructured":"SlotsJ.Herpesviruses in periodontal diseases.Periodontol 2000 2005:38:33\u201362.","DOI":"10.1111\/j.1600-0757.2005.00109.x"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2004.00816.x"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1079-2104(03)00352-4"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411980090040701"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0531-5565(03)00069-X"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1530\/eje.1.01874"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1180"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.11.1634"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.don.0000153839.74546.66"}],"container-title":["Oral Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1399-302X.2006.00268.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1399-302X.2006.00268.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T16:21:47Z","timestamp":1696868507000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1399-302X.2006.00268.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,2,13]]},"references-count":24,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2006,4]]}},"alternative-id":["10.1111\/j.1399-302X.2006.00268.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1399-302x.2006.00268.x","archive":["Portico"],"relation":{},"ISSN":["0902-0055","1399-302X"],"issn-type":[{"value":"0902-0055","type":"print"},{"value":"1399-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,2,13]]}}}