{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,13]],"date-time":"2026-11-13T09:55:45Z","timestamp":1794563745896,"version":"4.9.1"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,6]],"date-time":"2006-07-06T00:00:00Z","timestamp":1152144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Microbiology and Immunology"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:p><jats:bold>Introduction:\u2002<\/jats:bold> Cytokines are of major importance in periodontal disease progression. Interleukin\u201012 (IL\u201012) stimulates interferon\u2010<jats:italic>\u03b3<\/jats:italic> production by T helper type 1 (Th1) cells while IL\u201018 induces Th1 responses when present with IL\u201012 but Th2 responses in the absence of IL\u201012. IL\u20101<jats:italic>\u03b2<\/jats:italic> has been correlated with periodontal disease destruction. This study determined the local concentrations of these cytokines in sites of gingivitis and periodontitis.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Gingival crevicular fluid was collected from two sites in each of 10 gingivitis patients and from two gingivitis sites and two periodontitis sites from each of 10 periodontitis patients. Serum samples were also collected. IL\u20101<jats:italic>\u03b2<\/jats:italic>, biologically active IL\u201012 p70, the IL\u201012 p40 subunit and IL\u201018 concentrations were determined by enzyme\u2010linked immunoabsorbent assay.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> IL\u20101<jats:italic>\u03b2<\/jats:italic> and IL\u201018 concentrations were higher in the gingival crevicular fluid from periodontitis patients than in that from gingivitis patients; IL\u201018 concentrations were higher than those of IL\u20101<jats:italic>\u03b2<\/jats:italic>. Very little IL\u201012, either p40 or p70, was detected in the gingival crevicular fluid samples. In the serum, very low levels of cytokines were found. The level of serum IL\u201012 p40, however, was higher than in the fluid from periodontitis sites of periodontitis patients.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The local production of IL\u20101<jats:italic>\u03b2<\/jats:italic> and IL\u201018 in the gingival crevicular fluid increased with increasing inflammation and IL\u201018 was the predominant cytokine at both gingivitis and periodontitis sites. Very little IL\u201012 was detected with levels decreasing with increasing inflammation. These results suggest that there is an association between severity of periodontal disease and levels of IL\u20101, IL\u201012 and IL\u201018.<\/jats:p>","DOI":"10.1111\/j.1399-302x.2006.00292.x","type":"journal-article","created":{"date-parts":[[2006,7,7]],"date-time":"2006-07-07T12:48:55Z","timestamp":1152276535000},"page":"256-260","source":"Crossref","is-referenced-by-count":130,"title":["Interleukin\u20101 \u03b2, interleukin\u201012 and interleukin\u201018 levels in gingival fluid and serum of patients with gingivitis and periodontitis"],"prefix":"10.1111","volume":"21","author":[{"given":"A.","family":"Orozco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Gemmell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Bickel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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