{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:46:23Z","timestamp":1762865183588},"reference-count":19,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,8,21]],"date-time":"2006-08-21T00:00:00Z","timestamp":1156118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Microbiology and Immunology"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p><jats:bold>Background\/aims:\u2002<\/jats:bold>  <jats:italic>Peptostreptococcus micros<\/jats:italic> is a gram\u2010positive bacterium that has been associated with chronic periodontitis and endodontic infections. The aims of this study were to investigate the production of proteases and the acquisition of plasmin activity by rough and smooth morphotypes of <jats:italic>P. micros<\/jats:italic>. The contribution of these properties in the migration of bacteria through a reconstituted basement membrane was also evaluated.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Protease activities were determined using chromogenic and fluorogenic substrates as well as by zymography. Plasminogen binding activity was studied using an enzyme\u2010linked immunosorbent assay. The role of proteases and plasmin\u2010acquired activity in tissue penetration was investigated using Matrigel.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> The rough morphotype strains of <jats:italic>P. micros<\/jats:italic>, but not the smooth morphotype strains, were found to possess chymotrypsin\u2010like and gelatinase activities, both of which were inhibited by a serine protease inhibitor. By zymography, three gelatinase bands (165, 129, and 115\u2003kDa) were identified. Both morphotypes of <jats:italic>P. micros<\/jats:italic> can bind human plasminogen on their cell surface. Once bound to <jats:italic>P. micros<\/jats:italic>, plasminogen activators of bacterial (streptokinase) and human (urokinase) origins were found to activate plasminogen into plasmin. Our results also showed that plasmin activity can be acquired by <jats:italic>P. micros<\/jats:italic> following co\u2010incubation with human brain microvascular endothelial cells in culture. When non\u2010coated cells were used, the rough morphotype strain (HG1262), which possesses chymotrypsin\u2010like and gelatinase activities, showed a better capacity to penetrate a reconstituted basement membrane (Matrigel) than the smooth morphotype strain (HG1251). Penetration of the Matrigel by <jats:italic>P. micros<\/jats:italic> HG1262 was inhibited by the presence of a serine protease inhibitor. In addition, cells of <jats:italic>P. micros<\/jats:italic> with plasmin activity showed a significantly greater tissue penetration capacity.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Our study suggests that endogenous proteolytic activities of <jats:italic>P. micros<\/jats:italic> as well as plasmin\u2010acquired activity, may facilitate dissemination of bacterial cells to surrounding periodontal tissues and blood vessels.<\/jats:p>","DOI":"10.1111\/j.1399-302x.2006.00298.x","type":"journal-article","created":{"date-parts":[[2006,8,21]],"date-time":"2006-08-21T07:45:28Z","timestamp":1156146328000},"page":"319-325","source":"Crossref","is-referenced-by-count":21,"title":["Contribution of proteases and plasmin\u2010acquired activity in migration of <i>Peptostreptococcus micros<\/i> through a reconstituted basement membrane"],"prefix":"10.1111","volume":"21","author":[{"given":"D.","family":"Grenier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Bouclin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,8,21]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2000.71.9.1387"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80298-6"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.7.2478-2484.1995"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-43-4-787"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1998.tb00754.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-47-2-135"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.12.6439-6444.1999"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.6160-6163.2004"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0902-0055.2003.00116.x"},{"key":"e_1_2_6_11_2","first-page":"275","volume-title":"Oral bacterial ecology: the molecular basis","author":"Grenier D","year":"2000"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(99)70217-6"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.1.81"},{"key":"e_1_2_6_14_2","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1139\/w98-016","article-title":"Aminopeptidase activities in Peptostreptococcus spp. are statistically correlated to gelatin hydrolysis","volume":"44","author":"Ng J","year":"1998","journal-title":"Can J Microbiol"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0757.2000.2240108.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1992.tb00011.x"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-997-0042-1"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/00220345950740111201"},{"key":"e_1_2_6_20_2","doi-asserted-by":"crossref","first-page":"2717","DOI":"10.1128\/iai.56.10.2717-2722.1988","article-title":"Isolation of a chymotrypsin\u2010like enzyme from Treponema denticola","volume":"56","author":"Uitto VJ","year":"1988","journal-title":"Infect Immun"}],"container-title":["Oral Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1399-302X.2006.00298.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1399-302X.2006.00298.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T17:04:07Z","timestamp":1696871047000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1399-302X.2006.00298.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,8,21]]},"references-count":19,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["10.1111\/j.1399-302X.2006.00298.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1399-302x.2006.00298.x","archive":["Portico"],"relation":{},"ISSN":["0902-0055","1399-302X"],"issn-type":[{"value":"0902-0055","type":"print"},{"value":"1399-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,8,21]]}}}