{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T19:04:26Z","timestamp":1773860666504,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,10,24]],"date-time":"2006-10-24T00:00:00Z","timestamp":1161648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Microbiology and Immunology"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:p><jats:bold>Aims:\u2002<\/jats:bold> The aim of the present study was to determine the role of cyclic adenosine monophosphate (cAMP) on arginase activity in a murine macrophage cell line (RAW264.7 cells) stimulated with lipopolysaccharide (LPS) from <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Materials and methods:\u2002<\/jats:bold> The cells were treated with <jats:italic>A. actinomycetemcomitans<\/jats:italic> LPS for 24\u2003h. The effects of SQ22536 (an adenylyl cyclase inhibitor), ODQ (a guanylyl cyclase inhibitor), dibutyryl cAMP (a cAMP analog), 8\u2010bromo cyclic guanosine monophosphate (a cGMP analog), forskolin (an adenylyl cylase activator), and cycloheximide (a protein synthesis inhibitor) on arginase activity in <jats:italic>A. actinomycetemcomitans<\/jats:italic> LPS\u2010stimulated RAW264.7 cells were also determined. Arginase activity was assessed in LPS\u2010stimulated cells in the presence of 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX), siguazodan and rolipram [phosphodiesterase (PDE) inhibitors] as well as KT5720 [a protein kinase A (PKA) inhibitor].<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Arginase activity in <jats:italic>A. actinomycetemcomitans<\/jats:italic> LPS\u2010stimulated RAW264.7 cells was suppressed by SQ22536 but not ODQ. Enhancement of arginase activity was observed in the presence of cAMP analog or forskolin but not cGMP analog. Cycloheximide blocked arginase activity in the cells in the presence of cAMP analog or forskolin with or without <jats:italic>A. actinomycetemcomitans<\/jats:italic> LPS. IBMX augmented arginase activity in <jats:italic>A. actinomycetemcomitans<\/jats:italic> LPS\u2010stimulated cells. Rolipram (a PDE4 inhibitor) increased the levels of arginase activity higher than siguazodan (a PDE3 inhibitor) in the antigen\u2010stimulated cells. The effect of cAMP analog or forskolin on arginase activity in the presence or absence of <jats:italic>A. actinomycetemcomitans<\/jats:italic> LPS was blocked by the PKA inhibitor (KT5720).<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The results of the present study suggest that <jats:italic>A. actinomycetemcomitans<\/jats:italic> LPS may stimulate arginase activity in murine macrophages (RAW264.7 cells) in a cAMP\u2010PKA\u2010dependent pathway.<\/jats:p>","DOI":"10.1111\/j.1399-302x.2006.00300.x","type":"journal-article","created":{"date-parts":[[2006,10,24]],"date-time":"2006-10-24T16:11:46Z","timestamp":1161706306000},"page":"347-352","source":"Crossref","is-referenced-by-count":5,"title":["The role of cyclic\u2010AMP on arginase activity by a murine macrophage cell line (RAW264.7) stimulated with lipopolysaccharide from <i>Actinobacillus actinomycetemcomitans<\/i>"],"prefix":"10.1111","volume":"21","author":[{"given":"W.","family":"Sosroseno","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Musa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ravichandran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Fikri Ibrahim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. 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