{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:02:15Z","timestamp":1790308935237,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,10,24]],"date-time":"2006-10-24T00:00:00Z","timestamp":1161648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oral Microbiology and Immunology"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:p> <jats:italic>Prevotella intermedia<\/jats:italic>, a black\u2010pigmented, anaerobic, gram\u2010negative bacterium, is associated with various type of periodontitis. Antibiotic treatments via a systemic or local route have been reported as being useful for treating periodontal disease. The purpose of this study was to examine the effects of four antibiotics, tetracycline (TET), minocycline (MINO), doxycycline (DOXY) and ofloxacin (OFLX) on <jats:italic>P. intermedia<\/jats:italic> biofilms at minimum inhibitory concentrations (MIC) from one\u2010fold to 100\u2010fold. MICs were determined for planktonic cells. Biofilm formation was determined with the crystal violet stain method and the bioactivities in the biofilms were determined with the adenosine triphosphate (ATP) \u2010bioluminescent assay using a 96\u2010well culture plate. At one\u2010fold MIC, DOXY inhibited biofilm formation by <jats:italic>P. intermedia<\/jats:italic> ATCC 25611. Other antibiotics at one\u2010fold MIC had no effects on the biofilm formation of tested bacterial strains. In <jats:italic>P. intermedia<\/jats:italic> ATCC 25611 biofilms, all the antibiotics tested showed inhibitory activities at five\u2010 to 100\u2010fold MICs. In the biofilms of <jats:italic>P. intermedia<\/jats:italic> strains, except ATCC 25611, treated with three tetracycline antibiotics, the bioactivities were significantly increased, indicating the difficulties involved in designing antibiotic therapy for periodontal disease.<\/jats:p>","DOI":"10.1111\/j.1399-302x.2006.00305.x","type":"journal-article","created":{"date-parts":[[2006,10,24]],"date-time":"2006-10-24T16:11:46Z","timestamp":1161706306000},"page":"366-371","source":"Crossref","is-referenced-by-count":34,"title":["The effects of tetracycline, minocycline, doxycycline and ofloxacin on <i>Prevotella intermedia<\/i> 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