{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T04:54:54Z","timestamp":1770267294194,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,9,9]],"date-time":"2012-09-09T00:00:00Z","timestamp":1347148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:sec><jats:label\/><jats:p><jats:bold>To cite this article:<\/jats:bold> Mart\u00edn\u2010Mu\u00f1oz MF, Fortuni M, Caminoa M, Belver T, Quirce S, Caballero T. Anaphylactic reaction to probiotics. Cow\u2019s milk and hen\u2019s egg allergens in probiotic compounds. <jats:italic>Pediatr Allergy Immunol<\/jats:italic> 2012: <jats:bold>23<\/jats:bold>: 778\u2013784.<\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p><jats:bold>Background:\u2002<\/jats:bold> Probiotics are used in the treatment of allergic diseases. We investigated the safety of probiotics for subjects with food allergy.<\/jats:p><jats:p><jats:bold>Material and methods:\u2002<\/jats:bold> Labels of probiotics commercially available in Spain were examined to assess their content of cow\u2019s milk or hen\u2019s egg. Skin prick tests with these compounds (20\u2003mg\/ml) were performed in five children allergic to cow\u2019s milk, five children allergic to hen\u2019s white egg, and five control subjects non\u2010allergic to food. Three serum pools: I (positive\u2010specific IgE to cow\u2019s milk and hen\u2019s egg white proteins), II (positive\u2010specific IgE to cow\u2019s milk and negative to hen\u2019s egg white proteins), and III (negative\u2010specific IgE to cow\u2019s milk and positive to hen\u2019s egg white proteins) were used to detect cow\u2019s milk and hen\u2019s egg white allergens in probiotics. ImmunoCAP<jats:sup>\u00ae<\/jats:sup> (Phadia), in\u2010house ELISA, SDS\u2010PAGE immunoblotting, and inhibition studies of these assays were performed. Proteins were quantified by enzyme\u2010immunoassay.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Eleven probiotics were studied. No label advertised about egg content, eight labels warned about lactose, lactic acid or cow\u2019s milk, one label claimed to be milk\u2010free, and two gave no information. Cow\u2019s milk proteins were detected, by at least one lab technique, in 10\/11 probiotics, three over 2.5\u2003mg\/kg (21, 52, 112\u2003mg\/kg). Hen\u2019s egg white proteins were detected in 3\/11 probiotics, only one had more than 2.5\u2003mg\/kg (47\u2003mg\/kg).<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> Probiotic compounds may contain hidden allergens of food and may not be safe for subjects with allergy to cow\u2019s milk or hen\u2019s egg.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1399-3038.2012.01338.x","type":"journal-article","created":{"date-parts":[[2012,9,10]],"date-time":"2012-09-10T02:16:11Z","timestamp":1347243371000},"page":"778-784","source":"Crossref","is-referenced-by-count":41,"title":["Anaphylactic reaction to probiotics. 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